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Gut Dysbiosis during Influenza Contributes to Pulmonary Pneumococcal Superinfection through Altered Short-Chain Fatty Acid Production
Valentin Sencio
1
Adeline Barthélémy
1
Luciana Tavares
2
Marina Machado
1, 2
Daphnée Soulard
1
Céline Cuinat
3, 4
Celso Martins Queiroz-Junior
2
Marie-Louise Noordine
4, 3
Sophie Salomé-Desnoulez
1
Lucie Deryuter
1
Benoit Foligne
5
Céline Wahl
6
Benoit Frisch
7
Angelica Vieira
2
Christophe Paget
1
Graeme Milligan
8
Trond Ulven
9
Isabelle Wolowczuk
1
Christelle Faveeuw
1
Ronan Le Goffic
10
Muriel Thomas
4, 3
Stéphanie Ferreira
6
Mauro Teixeira
2
François Trottein
1, *
*
Corresponding author
Valentin Sencio 1
Author
Adeline Barthélémy 1
Author
Luciana Tavares 2
Author
Marina Machado 1, 2
Author
Daphnée Soulard 1
Author
Céline Cuinat 3, 4
Author
Celso Martins Queiroz-Junior 2
Author
Marie-Louise Noordine 4, 3
Mail :
Author
Sophie Salomé-Desnoulez 1
Author
Lucie Deryuter 1
Author
Benoit Foligne 5
Author
Céline Wahl 6
Author
Benoit Frisch 7
Mail :
Author
Angelica Vieira 2
Author
Christophe Paget 1
Author
Graeme Milligan 8
Author
Trond Ulven 9
Author
Isabelle Wolowczuk 1
Author
Christelle Faveeuw 1
Author
Ronan Le Goffic 10
Author
Muriel Thomas 4, 3
Author
Stéphanie Ferreira 6
Author
Mauro Teixeira 2
Mail :
Author
François Trottein 1
Mail :
Correspondent author IdHAL : francois-trottein ORCID : https://orcid.org/0000-0003-3373-1814
1
CIIL - Centre d’Infection et d’Immunité de Lille (CIIL) - U1019 - UMR 8204 (1 Rue du Professeur Calmette - Lille Cedex - 59019 - BP 245 - France)
- Institut Pasteur de Lille : UMR8204 (1 Rue du Professeur Calmette - Lille Cedex - 59019 - France)
- RIIP - Réseau International des Instituts Pasteur (25, rue du Dr Roux 75724 Paris Cedex 15 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1019 (101, rue de Tolbiac, 75013 Paris - France)
- Université de Lille : UMR9017 (Lille - France)
- CNRS - Centre National de la Recherche Scientifique : UMR9017 (France)
2
Instituto de Ciencias Biologicas [Minas Gerais] (Avenida Presidente Antônio Carlos, 6627 - Campus UFMG, Belo Horizonte - Minas Gerais, 31270-901 - Brazil)
- UFMG - Universidade Federal de Minas Gerais [Belo Horizonte] (Av. Antônio Carlos, 6627 - Pampulha - Belo Horizonte - MG - Brazil)
3
Université Paris-Saclay (Espace Technologique, Bat. Discovery - RD 128 - 2e ét., 91190 Saint-Aubin - France)
4
MICALIS - MICrobiologie de l'ALImentation au Service de la Santé (F-78350 JOUY-EN-JOSAS - France)
- AgroParisTech (16 rue Claude Bernard F-75231 Paris Cedex 05 - France)
- INRAE - Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement : UMR1319 (France)
5
LIRIC - Lille Inflammation Research International Center (Faculté de médecine
Pôle recherche-4ème étage
Place de Verdun
F-59045 Lille Cedex - France)
- Institut Pasteur de Lille : U995 (1 Rue du Professeur Calmette - Lille Cedex - 59019 - France)
- RIIP - Réseau International des Instituts Pasteur (25, rue du Dr Roux 75724 Paris Cedex 15 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U995 (101, rue de Tolbiac, 75013 Paris - France)
- Université de Lille (Lille - France)
- CHRU Lille - Centre Hospitalier Régional Universitaire [Lille] : U995 (2, avenue Oscar Lambret - 59037 Lille Cedex - France)
6
Genoscreen [Lille] (Campus de l'Institut Pasteur, 1 rue du Professeur Calmette, Lille 59000 - France)
- Institut Pasteur de Lille (1 Rue du Professeur Calmette - Lille Cedex - 59019 - France)
- RIIP - Réseau International des Instituts Pasteur (25, rue du Dr Roux 75724 Paris Cedex 15 - France)
7
LCB - Laboratoire de chimie bioorganique (FACULTE DE PHARMACIE 74 Route du Rhin - BP 24 67401 ILLKIRCH CEDEX - France)
- CNRS - Centre National de la Recherche Scientifique : UMR7514 (France)
8
Institute of Molecular, Cell and Systems Biology - Centre for Translational Pharmacology [Glasgow, Scotland, UK] (United Kingdom)
- University of Glasgow (Glasgow G12 8QQ Scotland - United Kingdom)
9
Department of Pharmacology [Copenhagen, Denmark] (Denmark)
- KU - University of Copenhagen = Københavns Universitet (Nørregade 10, 1165 København, Danemark - Denmark)
10
VIM - Unité de recherche Virologie et Immunologie Moléculaires (UR0892 INRA Centre de Recherche de Jouy-en-Josas F-78352 Jouy-en-Josas cedex - France)
- INRAE - Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement : UR0892 (France)
- UVSQ - Université de Versailles Saint-Quentin-en-Yvelines : UMR (55 avenue de Paris - 78035 Versailles cedex - France)
Abstract : Secondary bacterial infections often complicate viral respiratory infections. We hypothesize that perturbation of the gut microbiota during influenza A virus (IAV) infection might favor respiratory bacterial superinfection. Sublethal infection with influenza transiently alters the composition and fermentative activity of the gut microbiota in mice. These changes are attributed in part to reduced food consumption. Fecal transfer experiments demonstrate that the IAV-conditioned microbiota compromises lung defenses against pneumococcal infection. In mechanistic terms, reduced production of the predominant short-chain fatty acid (SCFA) acetate affects the bactericidal activity of alveolar macrophages. Following treatment with acetate, mice colonized with the IAV-conditioned microbiota display reduced bacterial loads. In the context of influenza infection, acetate supplementation reduces, in a free fatty acid receptor 2 (FFAR2)-dependent manner, local and systemic bacterial loads. This translates into reduced lung pathology and improved survival rates of double-infected mice. Lastly, pharmacological activation of the SCFA receptor FFAR2 during influenza reduces bacterial superinfection.
Document type :
Journal articles
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https://www.hal.inserm.fr/inserm-02501395
Contributor : François Trottein <>
Submitted on : Friday, March 6, 2020 - 6:41:11 PM
Last modification on : Monday, March 30, 2020 - 8:30:04 AM
Contributor : François Trottein <>
Submitted on : Friday, March 6, 2020 - 6:41:11 PM
Last modification on : Monday, March 30, 2020 - 8:30:04 AM
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211470.pdf
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Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives 4.0 International License
Identifiers
- HAL Id : inserm-02501395, version 1
- DOI : 10.1016/j.celrep.2020.02.013
- PUBMED : 32130898
Collections
RIIP | UVSQ | AGROPARISTECH | UNIV-LILLE | UNIV-PARIS-SACLAY | CNRS | RIIP_LILLE
Citation
Valentin Sencio, Adeline Barthélémy, Luciana Tavares, Marina Machado, Daphnée Soulard, et al.. Gut Dysbiosis during Influenza Contributes to Pulmonary Pneumococcal Superinfection through Altered Short-Chain Fatty Acid Production. Cell Reports , Elsevier Inc, 2020, 30 (9), pp.2934-2947.e6. ⟨10.1016/j.celrep.2020.02.013⟩. ⟨inserm-02501395⟩
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