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C9C5 positive mature oligodendrocytes are a source of Sonic Hedgehog in the mouse brain

Abstract : In the mature rodent brain, Sonic Hedgehog (Shh) signaling regulates stem and progenitor cell maintenance, neuronal and glial circuitry and brain repair. However, the sources and distribution of Shh mediating these effects are still poorly characterized. Here, we report in the adult mouse brain, a broad expression pattern of Shh recognized by the specific mono-clonal C9C5 antibody in a subset (11-12%) of CC1 + mature oligodendrocytes that do not express carbonic anhydrase II. These cells express also Olig2 and Sox10, two oligodendro-cyte lineage-specific markers, but not PDGFRα, a marker of oligodendrocyte progenitors. In agreement with oligodendroglial cells being a source of Shh in the adult mouse brain, we identify Shh transcripts by single molecule fluorescent in situ hybridization in a subset of cells expressing Olig2 and Sox10 mRNAs. These findings also reveal that Shh expression is more extensive than originally reported. The Shh-C9C5-associated signal labels the oligo-dendroglial cell body and decorates by intense puncta the processes. C9C5 + cells are distributed in a grid-like manner. They constitute small units that could deliver locally Shh to its receptor Patched expressed in GFAP + and S100β + astrocytes, and in HuC/D + neurons as shown in Ptc LacZ/+ reporter mice. Postnatally, C9C5 immunoreactivity overlaps the myelina-tion peak that occurs between P10 and P20 and is down regulated during ageing. Thus, our data suggest that C9C5 + CC1 + oligodendroglial cells are a source of Shh in the mouse post-natal brain.
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Submitted on : Wednesday, February 26, 2020 - 4:44:19 PM
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Tirou et al. PlosOne 2020.pdf
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Linda Tirou, Mariagiovanna Russo, Hélène Faure, Giuliana Pellegrino, Ariane Sharif, et al.. C9C5 positive mature oligodendrocytes are a source of Sonic Hedgehog in the mouse brain. PLoS ONE, Public Library of Science, 2020, 15 (2), pp.e0229362. ⟨10.1371/journal.pone.0229362⟩. ⟨inserm-02492142⟩

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