Skip to Main content Skip to Navigation
Journal articles

Immunologic and Clinical Effects of Injecting Mature Peptide- Loaded Dendritic Cells by Intralymphatic and Intranodal Routes in Metastatic Melanoma Patients

Abstract : Purpose: A phase I/II trial was conducted to evaluate clinical and immunologic responses after intralymphatic and intranodal injections of mature dendritic cells. Experimental design: Fourteen patients with a metastatic melanoma received matured dendritic cells, loaded with Melan-A/MART-1 and/or NA17-A peptides and keyhole limpet hemocyanin. The cells were matured overnight with Ribomunyl, a toll-like receptor ligand, and IFN-gamma, which ensured the production of high levels of interleukin-12p70. Dendritic cells were injected at monthly intervals, first into an afferent lymphatic and then twice intranodally. Immunologic responses were monitored by tetramer staining of circulating CD8(+) lymphocytes and delayed-type hypersensitivity tests. Results: Dendritic cell vaccination induced delayed-type hypersensitivity reactivity toward NA17-A-pulsed, keyhole limpet hemocyanin-pulsed, and Melan-A-pulsed dendritic cells in 6 of 10, 4 of 11, and 3 of 9 patients, respectively. Four of the 12 patients analyzed by tetramer staining showed a significantly increased frequency of Melan-A-specific T cells, including one patient vaccinated only with NA17-A-pulsed dendritic cells. Furthermore, 2 of the 12 analyzed patients had a significant increase of NA17-A-specific T cells, including one immunized after an optional additional treatment course. No objective clinical response was observed. Two patients were stabilized at 4 and 10 months and three patients are still alive at 30, 39, and 48 months. Conclusions: Injections into the lymphatic system of mature peptide-loaded dendritic cells with potential TH1 polarization capacities did not result in marked clinical results, despite immunologic responses in some patients. This highlights the need to improve our understanding of dendritic cell physiology.
Complete list of metadatas

Cited literature [39 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02483767
Contributor : Nathalie Labarriere <>
Submitted on : Tuesday, February 18, 2020 - 6:48:58 PM
Last modification on : Friday, February 21, 2020 - 1:25:35 AM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Collections

Citation

Thierry Lesimple, Eve-Marie Neidhard, Virginie Vignard, Claudia Lefeuvre, Henri Adamski, et al.. Immunologic and Clinical Effects of Injecting Mature Peptide- Loaded Dendritic Cells by Intralymphatic and Intranodal Routes in Metastatic Melanoma Patients. Clinical Cancer Research, American Association for Cancer Research, 2006, 12 (24), pp.7380-7388. ⟨10.1158/1078-0432.CCR-06-1879⟩. ⟨inserm-02483767⟩

Share

Metrics

Record views

107