Skip to Main content Skip to Navigation
Journal articles

Circulating Tumor Cell Detection and Polyomavirus Status in Merkel Cell Carcinoma

Abstract : The incidence of Merkel cell carcinoma (MCC), a rare and highly metastatic skin malignancy, has sharply increased in the last decade. Clinical biomarkers are urgently needed for MCC prognosis, treatment response monitoring, and early diagnosis of relapse. The clinical interest of circulating tumors cells (CTCs) has been validated in many solid cancers. The aim of this study was to compare CTC detection and characterization in blood samples of patients with MCC using the CellSearch System and the RosetteSep -DEPArray workflow, an innovative procedure to enrich, detect and isolate single CTCs. In preliminary experiments (using spiked MCC cell lines) both methods allowed detecting very few MCC cells. In blood samples from 19 patients with MCC at different stages, CellSearch detected MCC CTCs in 26% of patients, and the R-D workflow in 42% of patients. The detection of CTC-positive patients increased to 52% by the cumulative positivity rate of both methodologies. Moreover, Merkel cell polyomavirus DNA, involved in MCC oncogenesis, was detected in tumor biopsies, but not in all single CTCs from the same patient, reflecting the tumor heterogeneity. Our data demonstrate the possibility to detect, isolate and characterize CTCs in patients with MCC using two complementary approaches.
Complete list of metadatas

Cited literature [45 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02482735
Contributor : Myriam Bodescot <>
Submitted on : Tuesday, February 18, 2020 - 11:36:53 AM
Last modification on : Friday, May 15, 2020 - 12:22:11 PM

File

s41598-020-58572-9.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Magali Boyer, Laure Cayrefourcq, Françoise Garima, Vincent Foulongne, Olivier Dereure, et al.. Circulating Tumor Cell Detection and Polyomavirus Status in Merkel Cell Carcinoma. Scientific Reports, Nature Publishing Group, 2020, 10 (1), pp.1612. ⟨10.1038/s41598-020-58572-9⟩. ⟨inserm-02482735⟩

Share

Metrics

Record views

102

Files downloads

124