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Dominant TCR Valpha usage by virus and tumor- reactive T cells with wide affinity ranges for their specific antigens

Abstract : We have studied the TCR features and functional responses of three sets of human cytolytic T cell (CTL) clones, recognizing antigenic peptides presented by HLA-A2 and derived from the Epstein-Barr virus proteins BMLF1 and BRLF1 and from the melanoma protein Melan-A/MART-1. Within each set, a majority of clones used a recurrent V alpha region, even though they expressed highly diverse TCR beta chains and V(D)J junctional sequences. Functional assays and peptide/MHC multimer binding studies indicated that this restricted V alpha usage was not associated with the affinity/avidity of the CTL clones. The V alpha dominance, which may be a frequent feature of antigen-specific T cells, likely reflects a restricted geometry of TCR/peptide/MHC complexes, primarily determined by V alpha CDR.
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https://www.hal.inserm.fr/inserm-02482088
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Lydie Trautmann, Nathalie Labarrière, Francine Jotereau, Vaios Karanikas, Nadine Gervois, et al.. Dominant TCR Valpha usage by virus and tumor- reactive T cells with wide affinity ranges for their specific antigens. European Journal of Immunology, Wiley-VCH Verlag, 2002, 32 (11), pp.3181-3190. ⟨10.1002/1521-4141(200211)32:11<3181::AID-IMMU3181>3.0.CO;2-2⟩. ⟨inserm-02482088⟩

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