Skip to Main content Skip to Navigation
Journal articles

Apoptotic Body-Loaded Dendritic Cells Efficiently Cross-Prime Cytotoxic T Lymphocytes Specific for NA17-A Antigen but Not for Melan-A/MART-1 Antigen

Abstract : DCs hold promise for cancer immunotherapy due to their functional ambivalence: iDCs internalize antigens, then mDCs trigger naive T-cell activation. However, no consensus has been reached concerning the optimal mode of antigen acquisition for efficient cross-priming of TAA-specific CTLs, and this remains a field of investigation. Here, we used highly purified apobodies derived from an HLA-A*0201-negative melanoma line as a source of tumor antigens for HLA-A*0201 DCs. We compared in vitro mDCs loaded with apobodies to DCs loaded with antigenic peptides, NA17-A 1-9 and Melan-A/MART-1 26-35 A27L analogue, for their capacity to stimulate melanoma antigen-specific T cells from autologous PBLs. Apobody phagocytosis did not induce spontaneous DC maturation, but phagocytic DCs were still responsive to mat-uration signals, resulting in a functional ability to activate antigen-specific lymphocytes. NA17-A-specific T lympho-cytes were activated by both types of stimulation, whereas only peptide-pulsed DCs stimulated the growth of Melan-A/ MART-1-specific lymphocytes. We also observed a lack of staining of melanoma-derived apobodies with a Melan-A-specific MAb, suggesting protein alteration during apoptosis induction. After HLA-A*0201/NA17-A multimer sorting, an-tigen-specific lymphocytes induced by mature DCs loaded with either peptide or apobodies displayed similar functional capacity against peptide-pulsed T2 cells and melanoma cells. Therefore, apobody-loaded DCs can achieve T-cell priming similar to that induced by peptide-pulsed DCs, provided that the apoptotic process allows the preservation of antigen expression .
Complete list of metadatas

Cited literature [34 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02482082
Contributor : Nathalie Labarriere <>
Submitted on : Monday, February 17, 2020 - 6:25:42 PM
Last modification on : Friday, June 5, 2020 - 3:44:03 AM
Document(s) archivé(s) le : Monday, May 18, 2020 - 7:53:28 PM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Collections

Citation

Nathalie Labarrière, Laurent Bretaudeau, Nadine Gervois, Marie Bodinier, Gwenola Bougras, et al.. Apoptotic Body-Loaded Dendritic Cells Efficiently Cross-Prime Cytotoxic T Lymphocytes Specific for NA17-A Antigen but Not for Melan-A/MART-1 Antigen. Cancer Immunology, Immunotherapy, Springer Verlag, 2002, 101 (3), pp.280-286. ⟨10.1002/ijc.10605⟩. ⟨inserm-02482082⟩

Share

Metrics

Record views

77