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C. Schurkes, W. Brock, J. Abel, and K. Unfried, Induction of 8-hydroxydeoxyguanosine by man 1605 made vitreous fibres and crocidolite asbestos administered intraperitoneally in rats

D. A. Altomare, C. W. Menges, and J. Pei, Activated TNF-alpha/NF-kappaB signaling via 1608 down-regulation of Fas-associated factor 1 in asbestos-induced mesotheliomas from Arf 1609 knockout mice, Proc Natl Acad Sci U S A, vol.106, pp.3420-3425, 2009.

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Q. Chen, J. Marsh, B. Ames, and B. Mossman, Detection of 8-oxo-2'-deoxyguanosine, a marker 1614 of oxidative DNA damage, in culture medium from human mesothelial cells exposed to 1615 crocidolite asbestos, Carcinogen, vol.17, pp.2525-2527, 1996.

H. Fung, Y. W. Kow, B. Van-houten, and B. T. Mossman, Patterns of 8-hydroxydeoxyguanosine 1617 formation in DNA and indications of oxidative stress in rat and human pleural mesothelial 1618 cells after exposure to crocidolite asbestos, Carcinogenesis, vol.18, pp.825-832, 1997.

J. Nygren, S. Suhonen, H. Norppa, and K. Linnainmaa, DNA damage in bronchial epithelial and 1620 mesothelial cells with and without associated crocidolite asbestos fibers, Environ Mol, p.1621

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, Res, vol.169, pp.141-148, 1986.

A. Renier, F. Levy, F. Pilliere, and M. C. Jaurand, Uncheduled DNA synthesis in rat pleural 1626 mesothelial cells treated with mineral fibres or benzo[a]pyrene, Mutat Res, vol.241, pp.361-1627, 1990.

H. Fung, Y. W. Kow, and B. Van-houten, Asbestos increases mammalian AP-1629 endonuclease gene expression, protein levels, and enzyme activity in mesothelial cells

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C. L. Zanella, C. R. Timblin, and A. Cummins, Asbestos-induced phosphorylation of 1638 epidermal growth actor receptor is linked to c-fos and apoptosis, Am J, p.1639

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, Chapter ex15 new22Revised Vf(text and tables

, Figure 15-1. Multistage Development of Asbestos-Induced Mesothelioma

. Adapted and . Shukla, , vol.76, 2003.

. Nymark, , vol.253, 2008.

. Pacurari, , vol.114, 2010.

. Broaddus, , vol.254, 2011.

R. Libbus, 255] Rat, i.p. administration Chromosome analysis in MM. Crocidolite Chrysotile Loss of chromosomes X, 8, 16, Translocations involving 5, 10 and 13, vol.18, 1988.

. Ni, , p.256, 2000.

, Investigation of p53 (exons 5-8), and K-ras (exons 1, 2) mutations in MM

, Crocidolite No mutation detected

. Unfried, , 1997.

, Rat, i.p. administration. Investigation of p53 mutations in MM

, Crocidolite No mutation detected in p53 while numerous base substitution were found in B

. Unfried, , 2004.

, Big Blue rat, i.p. administration. Crocidolite Significantly enhanced mutation rate of lacI gene from omenta 12 and 24 weeks post-exposure*

. Schürkes, , 2004.

, Crocidolite Significantly enhanced level of 8-OHdG in DNA from omenta 10-20 weeks post treatment

M. Vaslet, , 2002.

. Fleury-feith, , 2003.

, Mice, N?2 heterozygous, i.p. administration. Gene analysis. Crocidolite LOH at the N?2 locus

A. , , 2005.

, N?2 heterozygous, i.p. administration. Gene analysis. Crocidolite LOH at the N?2 locus. Deletion INK4 locus

. Lecomte, , 2005.

A. , , 2009.

, Arf heterozygous, i.p. administration. Gene analysis. Crocidolite LOH at the Arf locus. Hemizygous loss of Faf1 (Fas-associated factor 1)

, A to T (6%), A to G and insertion (3%), intraperitoneal LOH : Loss Of Heterozygozity * G to T predominant (29%) followed by deletion (26%), G to A (20%), G to C (12%)

. Olofsson, , 1989.

, Karyotype analysis (G banding)

, Crocidolite Chrysotile Amosite Non random aneuploidy, deletion, translocations, inversions (but not breaks, dicentrics, fragments, polyploidization)

. Pelin, , vol.46, 1995.

. Burmeister, , 2004.

, Normal cells and human Met-5A

, DNA breakage (comet assay, quantification of DNA-strand breaks and Fpg-sensitive sites by alkaline unwinding*)

, Crocidolite Chrysotile DNA breakage in both assays, but no increase in Fpgsensitive sites. No effect on MeT-5A cells

. Poser, , 2004.

C. , , 1996.

. Met-5a, Formation of 8-oxo-2'-deoxyguanosine released in the culture medium (HPLC)

, Crocidolite Increased level of of 8-oxo-2'-deoxyguanosine

. Fung, MeT-5A. Formation of 8-OH-dG in DNA (HPLC), vol.263, 1997.

, Crocidolite Decreased level of 8-OH-dG

W. Jensen, , 1999.

. Met-5a, High-resolution time-lapse microscopy. Crocidolite Chrysotile Delayed cytokinesis. Formation of bi-multinucleated cells

. Nygren, , 2004.

, Crocidolite Increased DNA breakage, more pronounced in cells associated with fibers than in cells without fibers

R. Jaurand, Pleural mesothelial cells. Morphological study of metaphases. Chrysotile Increased chromosome breakage, vol.265, 1986.
URL : https://hal.archives-ouvertes.fr/inserm-02449474

. Achard, Sister chromatid exchanges. Crocidolite Increased sister chromatid exchanges, vol.42, 1987.

. Wang, Pleural mesothelial cells. Ultrastructural study of metaphases, vol.43, 1987.

, Crocidolite Chrysotile Polyploidization, chromosome deformities (vacuolization)

. Renier, 266] Pleural mesothelial cells. DNA repair (unscheluled DNA synthesis), 1990.

. Yegles, Pleural mesothelial cells. Morphological study of mitotic cells, vol.44, 1993.

. Dong, Pleural mesothelial cells. DNA repair (unscheluled DNA synthesis), vol.40, 1994.

, Crocidolite Chrysotile Increased DNA repair. Partial involvement of ROS

. Dong, 41] Pleural mesothelial cells. DNA repair (poly(ADP-ribose) synthesis), 1995.

, Crocidolite Chrysotile Increased DNA repair. Partial involvement of ROS

. Yegles, Pleural mesothelial cells. Morphological study of mitotic cells, vol.47, 1995.

, Crocidolite Chrysotile Amosite Induction of abnormal anaphases and telophases

. Fung, Pleural mesothelial cells. Formation of 8-OH-dG in DNA (HPLC) Crocidolite Increased level of 8-OH-dG, vol.263, 1997.

. Levresse, , vol.34, 1997.

. /m-accumulation, G0/G1 accumulation and timedependent p53 and p21 expression (chrysotile)

. Fung, , 1998.

, Pleural mesothelial cells, induction de l'enzyme apurinic/apyrimidinic endonuclease

, Crocidolite Increased level (mRNA and protein)

D. Crocidolite and . Breakage, Cell cyle arrest in G2/M. Phagocytosis reduction by cytochalasin reduces DNA breakage

, Met-5A : an SV40-transformed human mesothelial cell line

, ROS : Reactive Oxygen Species * Fpg protein, which recognizes oxidized bases such as 8-oxo-guanine, is used as indicative of oxidative DNA-base modifications

, Reduction of micronucleus formation by antioxidants (metal chelators and ROS scavengers). ROS produced by fibers (crocidolite), 1994.

, Pleural mesothelial cells from rat

, Crocidolite Chrysotile Increased mRNA expression of c-fos and c-jun

. Timblin, , 1998.

, Pleural mesothelial cells from rat. Crocidolite Increased mRNA and protein expression of c-fos and c-jun

. Zanella, , 1999.

, Crocidolite Increased expression of mRNA c-fos via enhancement of EGFR level

. Berken, , 2003.

A. , , 2009.

, Crocidolite Regulation of NF-?B dependent on Faf1 expression in response to TNF-?. Upregulated in cell showing loss of Faf1 (see Table, pp.22-23

, Met-5A : a SV40-transformed human mesothelial cell line