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Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy

Abstract : Three-dimensional live imaging has become an indispensable technique in the fields of cell, developmental and neural biology. Precise spatio-temporal manipulation of biological entities is often required for a deeper functional understanding of the underlying biological process. Here we present a home-built integrated framework and optical design that combines three-dimensional light-sheet imaging over time with precise spatio-temporal optical manipulations induced by short infrared laser pulses. We demonstrate their potential for sub-cellular ablation of neurons and nuclei, tissue cauterization and optogenetics by using the Drosophila melanogaster and zebrafish model systems.
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https://www.hal.inserm.fr/inserm-02477266
Contributor : Myriam Bodescot <>
Submitted on : Thursday, February 13, 2020 - 11:55:45 AM
Last modification on : Tuesday, May 26, 2020 - 6:50:46 PM

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Gustavo de Medeiros, Dimitri Kromm, Balint Balazs, Nils Norlin, Stefan Günther, et al.. Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy. Scientific Reports, Nature Publishing Group, 2020, 10 (1), pp.1942. ⟨10.1038/s41598-019-54349-x⟩. ⟨inserm-02477266⟩

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