Skip to Main content Skip to Navigation
Journal articles

Lipophilic Fluorochrome Trackers of Membrane Transfers between Immune Cells

Abstract : Cell-to-cell transfers of membrane molecules between lymphoid cells (sometimes referred to as trogocytosis) is frequent and has multiple physiological consequences. Although difficult to visualize through the tracking of defined cell surface proteins, this process can be readily monitored by inserting PKH or CellVue Maroon fluorochromes into the plasma membranes of donor cells. We discuss here parameters that determine its detection by a flow-cytometry-based in vitro assay and present examples of application, including time-lapse video-microscopy analysis of transfers at the immunological synapse. By combining detection of cell-to-cell transfer and of cell surface CD107, it is possible to discriminate lymphoid cells binding target cells with and without perforin release. This could prove useful for identifying cells that destruct known target cells in autoimmune pathologies.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-02472265
Contributor : Mary Poupot <>
Submitted on : Monday, February 10, 2020 - 10:09:41 AM
Last modification on : Tuesday, February 18, 2020 - 3:32:02 PM

Identifiers

Collections

Citation

Julie Gertner-Dardenne, Mary Poupot, Brian Gray, Jean-Jacques Fournié. Lipophilic Fluorochrome Trackers of Membrane Transfers between Immune Cells. Immunological Investigations, Taylor & Francis, 2007, 36 (5), pp.665-685. ⟨10.1080/08820130701674646⟩. ⟨inserm-02472265⟩

Share

Metrics

Record views

67