The gene expression profile of phosphoantigen-specific human γδ T lymphocytes is a blend of αβ T-cell and NK-cell signatures

Frédéric Pont; Julien Familiades; Sébastien Dejean; Séverine Fruchon; Delphine Cendron; Mary Poupot; Rémy Poupot; Fatima l'Faqihi-Olive; Nais Prade; Bernard Ycart; Jean-Jacques Fournié

doi:10.1002/eji.201141870

Journal articles

The gene expression profile of phosphoantigen-specific human γδ T lymphocytes is a blend of αβ T-cell and NK-cell signatures

Frédéric Pont ¹ Julien Familiades ¹ Sébastien Dejean ² Séverine Fruchon ³ Delphine Cendron ¹ Mary Poupot ¹ Rémy Poupot ³ Fatima l'Faqihi-Olive ³ Nais Prade ⁴ Bernard Ycart ⁵ Jean-Jacques Fournié ¹

1 CRCT - Centre de Recherches en Cancérologie de Toulouse

2 IMT - Institut de Mathématiques de Toulouse UMR5219

3 CPTP - Centre de Physiopathologie Toulouse Purpan ex IFR 30 et IFR 150

4 Laboratoire d'Hématologie [Purpan]

5 IPS - Inférence Processus Stochastiques

LJK - Laboratoire Jean Kuntzmann

Abstract : Global transcriptional technologies have revolutionised the study of lymphoid cell populations, but human γδ T lymphocytes specific for phosphoantigens remain far less deeply characterised by these methods despite the great therapeutic potential of these cells. Here we analyse the transcriptome of circulating TCRVγ+ γδ T cells isolated from healthy individuals, and their relation with those from other lymphoid cell subsets. We report that the gene signature of phosphoantigen-specific TCRVγ+ γδ T cells is a hybrid of those from αβ T and NK cells, with more 'NK-cell' genes than αβ T cells have and more 'T-cell' genes than NK cells. The expression profile of TCRVγ+ γδ T cells stimulated with phosphoantigen recapitulates their immediate physiological functions: Th1 cytokine, chemokine and cytotoxic activities reflect their high mitotic activity at later time points and do not indicate antigen-presenting functions. Finally, such hallmarks make the transcriptome of γδ T cells, whether resting or clonally expanding, clearly distinctive from that of NK/T or peripheral T-cell lymphomas of the γδ subtype.

Keywords : Gamma-delta Lymphocyte Lymphoma Microarray Phosphoantigen

Domain :

Life Sciences [q-bio] / Human health and pathology / Hematology

Mathematics [math] / Statistics [math.ST]

Statistics [stat] / Statistics Theory [stat.TH]

Complete list of metadatas

https://www.hal.inserm.fr/inserm-02472241
Contributor : Mary Poupot <>
Submitted on : Monday, February 10, 2020 - 10:02:13 AM
Last modification on : Saturday, March 28, 2020 - 1:12:44 AM

The gene expression profile of phosphoantigen-specific human γδ T lymphocytes is a blend of αβ T-cell and NK-cell signatures

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The gene expression profile of phosphoantigen-specific human γδ T lymphocytes is a blend of αβ T-cell and NK-cell signatures

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