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Journal articles
The gene expression profile of phosphoantigen-specific human γδ T lymphocytes is a blend of αβ T-cell and NK-cell signatures
Frédéric Pont
1
Julien Familiades
1
Sébastien Dejean
2
Séverine Fruchon
3
Delphine Cendron
1
Mary Poupot
1
Rémy Poupot
3
Fatima l'Faqihi-Olive
3
Nais Prade
4
Bernard Ycart
5
Jean-Jacques Fournié
1
Frédéric Pont 1
Author
Julien Familiades 1
Author
Sébastien Dejean 2
Mail :
Author IdHAL : sebastien-dejean IdRef : https://www.idref.fr/06889564X
Séverine Fruchon 3
Author
Nais Prade 4
Author
Bernard Ycart 5
Mail : Site :
Author
Jean-Jacques Fournié 1
Mail :
Author IdHAL : jean-jacques-fournie
1
CRCT - Centre de Recherches en Cancérologie de Toulouse (Centre de Recherches en Cancérologie de Toulouse
2 Avenue Hubert Curien
31037 Toulouse Cedex 1
FRANCE - France)
- UT3 - Université Toulouse III - Paul Sabatier (118 route de Narbonne - 31062 Toulouse - France)
- Université Fédérale Toulouse Midi-Pyrénées (41 Allée Jules Guesde, 31000 Toulouse - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1037 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : ERL5294 (France)
2
IMT - Institut de Mathématiques de Toulouse UMR5219 (UPS IMT, F-31062 Toulouse Cedex 9,
INSA Toulouse, F-31077 Toulouse,
France UT1, F-31042 Toulouse,
France UT2, F-31058 Toulouse,
Téléphone : 05.61.55.67.90 - France)
- INSA Toulouse - Institut National des Sciences Appliquées - Toulouse (135, avenue de Rangueil - 31077 Toulouse cedex 4 - France)
- UT1 - Université Toulouse 1 Capitole (2 rue du Doyen-Gabriel-Marty - 31042 Toulouse Cedex 9 - France)
- UT2J - Université Toulouse - Jean Jaurès (5 allées Antonio Machado - 31058 Toulouse Cedex 9 - France)
- UT3 - Université Toulouse III - Paul Sabatier (118 route de Narbonne - 31062 Toulouse - France)
- Université Fédérale Toulouse Midi-Pyrénées (41 Allée Jules Guesde, 31000 Toulouse - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5219 (France)
3
CPTP - Centre de Physiopathologie Toulouse Purpan ex IFR 30 et IFR 150 (Hôpital de Purpan Place du Docteur Baylac 31024 TOULOUSE CEDEX 3 - France)
- UT3 - Université Toulouse III - Paul Sabatier (118 route de Narbonne - 31062 Toulouse - France)
- Université Fédérale Toulouse Midi-Pyrénées (41 Allée Jules Guesde, 31000 Toulouse - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1043 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5282 (France)
4
Laboratoire d'Hématologie [Purpan] (Pôle Biologie, Place du Docteur Baylac - TSA 40031 - 31059 Toulouse cedex 9 - France)
- UT3 - Université Toulouse III - Paul Sabatier (118 route de Narbonne - 31062 Toulouse - France)
- Université Fédérale Toulouse Midi-Pyrénées (41 Allée Jules Guesde, 31000 Toulouse - France)
- CHU Toulouse [Toulouse] (Hôtel-Dieu Saint Jacques 2, rue Viguerie TSA 80035 31059 Toulouse cedex 9 - France)
- Hôpital Purpan [Toulouse] (Place du Docteur Baylac
TSA 40031
31059 Toulouse cedex 9 - France)
- CHU Toulouse [Toulouse] (Hôtel-Dieu Saint Jacques 2, rue Viguerie TSA 80035 31059 Toulouse cedex 9 - France)
5
IPS - Inférence Processus Stochastiques (France)
- LJK - Laboratoire Jean Kuntzmann (Bâtiment IMAG, CS 40700, F-38058 Grenoble Cedex 9 - France)
- UPMF - Université Pierre Mendès France - Grenoble 2 (BP 47 - 38040 Grenoble Cedex 9 - France)
- UJF - Université Joseph Fourier - Grenoble 1 (BP 53 - 38041 Grenoble Cedex 9 - France)
- Grenoble INP [2007-2019] - Institut polytechnique de Grenoble - Grenoble Institute of Technology [2007-2019] (46 avenue Félix Viallet 38031 Grenoble Cedex 1 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5224 (France)
Abstract : Global transcriptional technologies have revolutionised the study of lymphoid cell populations, but human γδ T lymphocytes specific for phosphoantigens remain far less deeply characterised by these methods despite the great therapeutic potential of these cells. Here we analyse the transcriptome of circulating TCRVγ+ γδ T cells isolated from healthy individuals, and their relation with those from other lymphoid cell subsets. We report that the gene signature of phosphoantigen-specific TCRVγ+ γδ T cells is a hybrid of those from αβ T and NK cells, with more 'NK-cell' genes than αβ T cells have and more 'T-cell' genes than NK cells. The expression profile of TCRVγ+ γδ T cells stimulated with phosphoantigen recapitulates their immediate physiological functions: Th1 cytokine, chemokine and cytotoxic activities reflect their high mitotic activity at later time points and do not indicate antigen-presenting functions. Finally, such hallmarks make the transcriptome of γδ T cells, whether resting or clonally expanding, clearly distinctive from that of NK/T or peripheral T-cell lymphomas of the γδ subtype.
Document type :
Journal articles
Complete list of metadatas
https://www.hal.inserm.fr/inserm-02472241
Contributor : Mary Poupot <>
Submitted on : Monday, February 10, 2020 - 10:02:13 AM
Last modification on : Thursday, July 9, 2020 - 9:44:53 AM
Contributor : Mary Poupot <>
Submitted on : Monday, February 10, 2020 - 10:02:13 AM
Last modification on : Thursday, July 9, 2020 - 9:44:53 AM
Identifiers
- HAL Id : inserm-02472241, version 1
- DOI : 10.1002/eji.201141870
Collections
UNIV-TLSE3 | UGA | UT1-CAPITOLE | UNIV-TLSE2 | IMT | LJK | CNRS | INSMI | LJK_PS | LJK_PS_IPS | INSA-TOULOUSE | INSA-GROUPE
Citation
Frédéric Pont, Julien Familiades, Sébastien Dejean, Séverine Fruchon, Delphine Cendron, et al.. The gene expression profile of phosphoantigen-specific human γδ T lymphocytes is a blend of αβ T-cell and NK-cell signatures. European Journal of Immunology, Wiley-VCH Verlag, 2012, 42 (1), pp.228-240. ⟨10.1002/eji.201141870⟩. ⟨inserm-02472241⟩
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