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, Supplemental Figure S3 TMEM240 expression in the mouse brain

, Scale bars: 200 ?m for the hippocampal formation and caudoputamen and 100 ?m for the retrospenial area. TMEM240 immunoreactivity detected by immunofluorescence labeling (TMEM240/63-77, in green) in the hippocampal formation (d), caudoputamen (e), and retrospenial area of the cerebral cortex (f), Cp: caudoputamen, Dg: dentate gyrus, Re Sp: retrosplenial area, SCs: superior colliculus sensory related area

, Double immunofluorescence labeling of TMEM240 (TMEM240/63-77, in red) and calbindin

, Scale bar: 500 ?m. (b1-b3) Magnification of double immunofluorescence labeling image of TMEM240

, red) and calbindin (in green) in sagittal cerebellar sections. TMEM240-positive cells corresponding to the labeling of basket cells (1) and stellate cells (2), Scale bar: 50 ?m. Dn: dentate nucleus, FT: cerebellar fiber tracts, ML: molecular layer, GL: granular layer, PL: Purkinje-cell layer

, Supplemental Figure S6 Expression of TMEM240 in interneurons

, Scale bar: 50 ?m (b) Magnification of DNA labeling using DAPI. (c) Magnification of parvalbumin labeling (in green) in Purkinje and molecular layers. (d) Magnification of TMEM240 labeling (Santa Cruz Biotechnology, in red) in Purkinje and molecular layers. (e) Magnification of double labeling of TMEM240 and parvalbumin. The white arrow indicates basket cells, the grey arrow stellate cells, and the black arrow an excitatory neuron in the molecular layer, Double immunofluorescence labeling of TMEM240 (Santa Cruz Biotechnology, in red) and parvalbumin (in green) in sagittal cerebellar sections