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A functional autophagy pathway is required for rapamycin-induced degradation of the Sgs1 helicase in Saccharomyces cerevisiae

Abstract : In yeast Saccharomyces cerevisiae, the immunosuppressant rapamycin mimics starvation by inhibiting the kinase Tor1. We recently documented that this treatment triggers a rapid degradation of Sgs1, a helicase involved in several biological processes such as the prevention of genomic instability. Herein, we show that yeast strains deleted for genes ATG2, ATG9, and PEP4, encoding components of the autophagy pathway, prevent rapamycin-induced degradation of Sgs1. We propose that defects in the autophagy pathway prevent degradation of key proteins in the rapamycin response pathway and as a consequence cause resistance to the drug.
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https://www.hal.inserm.fr/inserm-02458189
Contributor : Sylvie Le Bihan <>
Submitted on : Tuesday, January 28, 2020 - 3:25:35 PM
Last modification on : Friday, January 31, 2020 - 1:27:44 AM

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Rim Marrakchi, Chedly Chouchani, Jérémie Poschmann, Emil Andreev, Mohamed Cherif, et al.. A functional autophagy pathway is required for rapamycin-induced degradation of the Sgs1 helicase in Saccharomyces cerevisiae. Biochemistry and Cell Biology, NRC Research Press, 2013, 91 (3), pp.123-130. ⟨10.1139/bcb-2012-0084⟩. ⟨inserm-02458189⟩

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