Skip to Main content Skip to Navigation

Qualitative differences in T‐cell activation by dendritic cell‐derived extracellular vesicle subtypes

Abstract : Exosomes, nano-sized secreted extracellular vesicles (EVs), are actively studied for their diagnostic and therapeutic potential. In particular, exosomes secreted by dendritic cells (DCs) have been shown to carry MHC-peptide complexes allowing efficient activation of T lymphocytes, thus displaying potential as promoters of adaptive immune responses. DCs also secrete other types of EVs of different size, subcellular origin and protein composition, whose immune capacities have not been yet compared to those of exosomes. Here, we show that large EVs (lEVs) released by human DCs are as efficient as small EVs (sEVs), including exosomes, to induce CD4+ T-cell activation in vitro When released by immature DCs, however, lEVs and sEVs differ in their capacity to orient T helper (Th) cell responses, the former favouring secretion of Th2 cytokines, whereas the latter promote Th1 cytokine secretion (IFN-γ). Upon DC maturation, however, these functional differences are abolished, and all EVs become able to induce IFN-γ. Our results highlight the need to comprehensively compare the functionalities of EV subtypes in all patho/physiological systems where exosomes are claimed to perform critical roles.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-02450851
Contributor : Clotilde Thery <>
Submitted on : Thursday, January 23, 2020 - 10:28:14 AM
Last modification on : Friday, March 27, 2020 - 2:43:18 AM

Links full text

Identifiers

Collections

Citation

Mercedes Tkach, Joanna Kowal, Andres Zucchetti, Lotte Enserink, Mabel Jouve, et al.. Qualitative differences in T‐cell activation by dendritic cell‐derived extracellular vesicle subtypes. EMBO Journal, EMBO Press, 2017, 36 (20), pp.3012-3028. ⟨10.15252/embj.201696003⟩. ⟨inserm-02450851⟩

Share

Metrics

Record views

59