P. Wolf, The nature and significance of platelet products in human plasma, Br. J. Haematol, vol.13, pp.269-288, 1967.

H. C. Anderson, Vesicles associated with calcification in the matrix of epiphyseal cartilage, J. Cell Biol, vol.41, pp.59-72, 1969.

N. Crawford, The presence of contractile proteins in platelet microparticles isolated from human and animal platelet-free plasma, 1971.

, Br. J. Haematol, vol.21, pp.53-69

A. J. Dalton, Microvesicles and vesicles of multivesicular bodies versus 'virus-like' particles, J. Natl Cancer Inst, vol.54, pp.1137-1148, 1975.

E. G. Trams, C. J. Lauter, S. Jr, N. Heine, and U. , Exfoliation of membrane ecto-enzymes in the form of micro-vesicles, Biochim. Biophys. Acta, vol.645, pp.63-70, 1981.

D. D. Taylor, H. D. Homesley, and G. J. Doellgast, Binding of specific peroxidase-labeled antibody to placentaltype phosphatase on tumor-derived membrane fragments, Cancer Res, vol.40, pp.4064-4069, 1980.

B. T. Pan, K. Teng, C. Wu, M. Adam, and R. M. Johnstone, Electron microscopic evidence for externalization of the transferrin receptor in vesicular form in sheep reticulocytes, J. Cell Biol, vol.101, pp.942-948, 1985.

D. D. Poutsiaka, E. W. Schroder, D. D. Taylor, E. M. Levy, and P. H. Black, Membrane vesicles shed by murine melanoma cells selectively inhibit the expression of Ia antigen by macrophages, J. Immunol, vol.134, pp.138-144, 1985.

C. Harding, J. Heuser, and P. Stahl, Receptormediated endocytosis of transferrin and recycling of the transferrin receptor in rat reticulocytes, J. Cell Biol, vol.97, pp.329-339, 1983.

R. M. Johnstone, M. Adam, J. R. Hammond, L. Orr, and C. Turbide, Vesicle formation during reticulocyte maturation. Association of plasma membrane activities with released vesicles (exosomes), J. Biol. Chem, vol.262, pp.9412-9420, 1987.

S. Kassis, C. J. Lauter, M. Stojanov, S. Jr, and N. , Exfoliation of the b-adrenergic receptor and the regulatory components of adenylate cyclase by cultured rat glioma C6 cells, Biochim. Biophys. Acta, vol.886, issue.86, pp.90184-90193, 1986.

G. Raposo, D. Tenza, S. Mecheri, R. Peronet, C. Bonnerot et al., Accumulation of major histocompatibility complex class II molecules in mast cell secretory granules and their release upon degranulation, Mol. Biol. Cell, vol.8, pp.2631-2645, 1997.

L. Zitvogel, A. Regnault, A. Lozier, J. Wolfers, C. Flament et al., Eradication of established murine tumors using a novel cell-free vaccine: dendritic cell-derived exosomes, Nat. Med, vol.4, pp.594-600, 1998.

J. Lotvall, Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles, J. Extracell. Vesicles, vol.3, 2014.

J. Klumperman and G. Raposo, The complex ultrastructure of the endolysosomal system, Cold Spring. Harb. Perspect. Biol, vol.6, 2014.

C. Thery, S. Amigorena, G. Raposo, and C. A. , Isolation and characterization of exosomes from cell culture supernatants and biological fluids, Curr. Protoc. Cell Biol. Chapter, pp.3-22, 2006.

C. Gardiner, D. Vizio, D. Sahoo, S. Thery, C. Witwer et al., Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey, J. Extracell. Vesicles, vol.5, p.32945, 2016.

E. Consortium, EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research, Nat. Methods, vol.14, pp.228-232, 2017.

A. Bobrie, M. Colombo, S. Krumeich, G. Raposo, and C. Thery, Diverse subpopulations of vesicles secreted by different intracellular mechanisms are present in exosome preparations obtained by differential ultracentrifugation, J. Extracell. Vesicles, vol.1, p.18397, 2012.

M. Ostrowski, Rab27a and Rab27b control different steps of the exosome secretion pathway, Nat. Cell Biol, vol.12, pp.19-30, 2010.

A. Bobrie, S. Krumeich, F. Reyal, C. Recchi, L. F. Moita et al., Rab27a supports exosome-dependent and -independent mechanisms that modify the tumor microenvironment and can promote tumor progression, Cancer Res, vol.72, pp.4920-4930, 2012.

J. Kowal, M. Tkach, and C. Thery, Biogenesis and secretion of exosomes, Curr. Opin. Cell Biol, vol.29, pp.116-125, 2014.
URL : https://hal.archives-ouvertes.fr/inserm-02452742

M. Colombo, G. Raposo, and C. Thery, Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles, Annu. Rev. Cell Dev. Biol, vol.30, pp.255-289, 2014.
URL : https://hal.archives-ouvertes.fr/inserm-02452769

N. Chaput and C. Thery, Exosomes: immune properties and potential clinical implementations, Semin. Immunopathol, vol.33, pp.419-440, 2011.

M. Colombo, Analysis of ESCRT functions in exosome biogenesis, composition and secretion highlights the heterogeneity of extracellular vesicles, J. Cell Sci, vol.126, pp.5553-5565, 2013.

J. Kowal, Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes, Proc. Natl Acad. Sci. USA, vol.113, pp.968-977, 2016.
URL : https://hal.archives-ouvertes.fr/inserm-02450678

M. F. Baietti, Syndecan-syntenin -ALIX regulates the biogenesis of exosomes, Nat. Cell Biol, vol.14, pp.677-685, 2012.

S. Keerthikumar, Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes, Oncotarget, vol.6, pp.375-390, 2015.

V. R. Minciacchi, Large oncosomes contain distinct protein cargo and represent a separate functional class of tumor-derived extracellular vesicles, Oncotarget, vol.6, pp.327-338, 2015.

E. Willms, Cells release subpopulations of exosomes with distinct molecular and biological properties, Sci. Rep, vol.6, p.22519, 2016.

R. Xu, D. W. Greening, A. Rai, H. Ji, and R. J. Simpson, Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct, Methods, vol.87, pp.11-25, 2015.

D. J. Clark, W. E. Fondrie, A. Yang, and L. Mao, Triple SILAC quantitative proteomic analysis reveals differential abundance of cell signaling proteins between normal and lung cancer-derived exosomes, J. Proteomics, vol.133, pp.161-169, 2016.

R. A. Haraszti, High-resolution proteomic and lipidomic analysis of exosomes and microvesicles from different cell sources, J. Extracell. Vesicles, vol.5, p.32570, 2016.

B. J. Tauro, D. W. Greening, R. A. Mathias, S. Mathivanan, H. Ji et al., Two distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids, Mol. Cell rstb.royalsocietypublishing.org Phil. Trans. R. Soc. B, vol.373, p.20160479, 2013.

, Proteomics, vol.12, pp.587-598

V. R. Minciacchi, MYC mediates large oncosome-induced fibroblast reprogramming in prostate cancer, Cancer Res, vol.77, pp.2306-2317, 2017.

M. Tkach, Qualitative differences in T-cell activation by dendritic cell-derived extracellular vesicle subtypes, EMBO J, vol.36, pp.3012-3028, 2017.
URL : https://hal.archives-ouvertes.fr/inserm-02450851

V. Muralidharan-chari, J. Clancy, C. Plou, M. Romao, P. Chavrier et al., ARF6-regulated shedding of tumor cell-derived plasma membrane microvesicles, Curr. Biol, vol.19, pp.1875-1885, 2009.

S. M. Johnson, C. Dempsey, C. Parker, A. Mironov, H. Bradley et al., Acute lymphoblastic leukaemia cells produce large extracellular vesicles containing organelles and an active cytoskeleton, J. Extracell. Vesicles, vol.6, p.1294339, 2017.

M. B. Headley, A. Bins, A. Nip, E. W. Roberts, M. R. Looney et al., Visualization of immediate immune responses to pioneer metastatic cells in the lung, Nature, vol.531, pp.513-517, 2016.

C. Gardiner, Y. J. Ferreira, R. A. Dragovic, C. W. Redman, and I. L. Sargent, Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis, 2013.

J. , , vol.2, 19671.

R. Vogel, A standardized method to determine the concentration of extracellular vesicles using tunable resistive pulse sensing, J. Extracell. Vesicles, vol.5, p.31242, 2016.

X. Osteikoetxea, Improved characterization of EV preparations based on protein to lipid ratio and lipid properties, PLoS ONE, vol.10, 2015.

L. Cicero and A. , Exosomes released by keratinocytes modulate melanocyte pigmentation, Nat. Commun, vol.6, 2015.

J. Ratajczak, K. Miekus, M. Kucia, J. Zhang, R. Reca et al., Embryonic stem cellderived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery, Leukemia, vol.20, pp.847-856, 2006.

H. Valadi, K. Ekstrom, A. Bossios, M. Sjostrand, J. J. Lee et al., Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells, Nat. Cell Biol, vol.9, pp.654-659, 2007.

J. D. Arroyo, Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma, Proc. Natl Acad. Sci. USA, vol.108, pp.5003-5008, 2011.

K. C. Vickers, B. T. Palmisano, B. M. Shoucri, R. D. Shamburek, and A. T. Remaley, MicroRNAs are transported in plasma and delivered to recipient cells by highdensity lipoproteins, Nat. Cell Biol, vol.13, pp.423-433, 2011.

J. R. Chevillet, Quantitative and stoichiometric analysis of the microRNA content of exosomes, Proc. Natl Acad. Sci. USA, vol.111, pp.888-902, 2014.

J. Palma, MicroRNAs are exported from malignant cells in customized particles, Nucleic Acids Res, vol.40, pp.9125-9185, 2012.

H. Ji, M. Chen, D. W. Greening, W. He, A. Rai et al., Deep sequencing of RNA from three different extracellular vesicle (EV) subtypes released from the human LIM1863 colon cancer cell line uncovers distinct Mirna-enrichment signatures, PLoS ONE, vol.9, 2014.

J. P. Tosar, F. Gambaro, J. Sanguinetti, B. Bonilla, K. W. Witwer et al., Assessment of small RNA sorting into different extracellular fractions revealed by high-throughput sequencing of breast cell lines, Nucleic Acids Res, vol.43, pp.5601-5616, 2015.
URL : https://hal.archives-ouvertes.fr/pasteur-01881321

C. Lasser, Two distinct extracellular RNA signatures released by a single cell type identified by microarray and next-generation sequencing, RNA Biol, vol.14, pp.58-72, 2017.

J. P. Tosar, A. Cayota, E. Eitan, M. K. Halushka, and K. W. Witwer, Ribonucleic artefacts: are some extracellular RNA discoveries driven by cell culture medium components?, J. Extracell. Vesicles, vol.6, p.1272832, 2017.

B. Mateescu, Obstacles and opportunities in the functional analysis of extracellular vesicle RNA-an ISEV position paper, J. Extracell. Vesicles, vol.6, p.1286095, 2017.

, rstb.royalsocietypublishing.org Phil. Trans. R. Soc. B, vol.373, p.20160479