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Journal articles
Piezo1‐dependent stretch‐activated channels are inhibited by Polycystin‐2 in renal tubular epithelial cells
Rémi Peyronnet
1
Joana Martins
1
Fabrice Duprat
1
Sophie Demolombe
1
Malika Arhatte
1
Martine Jodar
1
Michel Tauc
2
Christophe Duranton
2
Marc Paulais
3
Jacques Teulon
3
Eric Honoré
1
Amanda Patel
1
Malika Arhatte 1
Author
Martine Jodar 1
Author
Michel Tauc 2
Mail :
Author IdHAL : michel-tauc ResearcherId : http://www.researcherid.com/rid/F-1530-2013 ORCID : https://orcid.org/0000-0002-8383-9818
Christophe Duranton 2
Author
Marc Paulais 3
Author
Jacques Teulon 3
Mail :
Author
Eric Honoré 1
Mail :
Author
Amanda Patel 1
Author
1
IPMC - Institut de pharmacologie moléculaire et cellulaire (CNRS-IPMC 660 Route des lucioles 06560 VALBONNE - France)
- UNS - Université Nice Sophia Antipolis (... - 2019) (Parc Valrose - BP 2135 - 06103 Nice cedex 2 - France)
- UCA - Université Côte d'Azur (Parc Valrose, 28 avenue Valrose 06108 Nice Cedex 2 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR7275 (France)
2
LP2M - Laboratoire de PhysioMédecine Moléculaire (Parc Valrose 06108 Nice Cedex 2 - France)
- UNS - Université Nice Sophia Antipolis (... - 2019) (Parc Valrose - BP 2135 - 06103 Nice cedex 2 - France)
- UCA - Université Côte d'Azur (Parc Valrose, 28 avenue Valrose 06108 Nice Cedex 2 - France)
- CNRS - Centre National de la Recherche Scientifique : FRE3472 (France)
3
CRC (UMR_S 872) - Centre de Recherche des Cordeliers (CRBM des Cordeliers 15, rue de l'ecole de medecine batiment E 75270 Paris cedex 06 - France)
- UPMC - Université Pierre et Marie Curie - Paris 6 (4 place Jussieu - 75005 Paris - France)
- UPD5 - Université Paris Descartes - Paris 5 : U872 (12, rue de l'École de Médecine - 75270 Paris cedex 06 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U872 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR 872 (France)
Abstract : Mechanical forces associated with fluid flow and/or circumferential stretch are sensed by renal epithelial cells and contribute to both adaptive or disease states. Non-selective stretch-activated ion channels (SACs), characterized by a lack of inactivation and a remarkably slow deactivation, are active at the basolateral side of renal proximal convoluted tubules. Knockdown of Piezo1 strongly reduces SAC activity in proximal convoluted tubule epithelial cells. Similarly, overexpression of Polycystin-2 (PC2) or, to a greater extent its pathogenic mutant PC2-740X, impairs native SACs. Moreover, PC2 inhibits exogenous Piezo1 SAC activity. PC2 coimmunoprecipitates with Piezo1 and deletion of its N-terminal domain prevents both this interaction and inhibition of SAC activity. These findings indicate that renal SACs depend on Piezo1, but are critically conditioned by PC2.
Complete list of metadatas
https://www.hal.inserm.fr/inserm-02448127
Contributor : Marc Paulais <>
Submitted on : Wednesday, January 22, 2020 - 9:43:06 AM
Last modification on : Friday, March 27, 2020 - 2:33:41 AM
Contributor : Marc Paulais <>
Submitted on : Wednesday, January 22, 2020 - 9:43:06 AM
Last modification on : Friday, March 27, 2020 - 2:33:41 AM
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Identifiers
- HAL Id : inserm-02448127, version 1
- DOI : 10.1038/embor.2013.170
- PUBMED : 24157948
- PUBMEDCENTRAL : PMC3981085
Collections
UPMC | UNIV-PARIS5 | UNICE | UNIV-COTEDAZUR | SORBONNE-UNIVERSITE | CORDELIERS | USPC | CNRS | SU-SCIENCES
Citation
Rémi Peyronnet, Joana Martins, Fabrice Duprat, Sophie Demolombe, Malika Arhatte, et al.. Piezo1‐dependent stretch‐activated channels are inhibited by Polycystin‐2 in renal tubular epithelial cells. EMBO Reports, EMBO Press, 2013, 14 (12), pp.1143-1148. ⟨10.1038/embor.2013.170⟩. ⟨inserm-02448127⟩
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