Phosphatidylinositol (4,5)-bisphosphate dynamically regulates the K2P background K+ channel TASK-2 - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue Scientific Reports Année : 2017

Phosphatidylinositol (4,5)-bisphosphate dynamically regulates the K2P background K+ channel TASK-2

Résumé

Two-pore domain K2P K+ channels responsible for the background K+ conductance and the resting membrane potential, are also finely regulated by a variety of chemical, physical and physiological stimuli. Hormones and transmitters acting through Gq protein-coupled receptors (GqPCRs) modulate the activity of various K2P channels but the signalling involved has remained elusive, in particular whether dynamic regulation by membrane PI(4,5)P2, common among other classes of K+ channels, affects K2P channels is controversial. Here we show that K2P K+ channel TASK-2 requires PI(4,5)P2 for activity, a dependence that accounts for its run down in the absence of intracellular ATP and its full recovery by addition of exogenous PI(4,5)P2, its inhibition by low concentrations of polycation PI scavengers, and inhibition by PI(4,5)P2 depletion from the membrane. Comprehensive mutagenesis suggests that PI(4,5)P2 interaction with TASK-2 takes place at C-terminus where three basic aminoacids are identified as being part of a putative binding site.
Fichier principal
Vignette du fichier
srep45407.pdf (4.41 Mo) Télécharger le fichier
Loading...

Dates et versions

inserm-02448112 , version 1 (22-01-2020)

Identifiants

Citer

María Isabel Niemeyer, L. Pablo Cid, Marc Paulais, Jacques Teulon, Francisco Sepúlveda. Phosphatidylinositol (4,5)-bisphosphate dynamically regulates the K2P background K+ channel TASK-2. Scientific Reports, 2017, 7, pp.45407. ⟨10.1038/srep45407⟩. ⟨inserm-02448112⟩
319 Consultations
341 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More