RNAs were retro-transcribed using Superscript transcriptase (Superscript II, Invitrogen). Following forward and reverse primers were used, extraction and quantitative real-time PCR. Total RNAs were extracted with RNAeasy mini kit, p.48 ,
, Protein extracts were separated by SDS-PAGE, transferred onto a PVDF membrane (Millipore, Billerica, MA, USA) and revealed with a chemiluminescence kit (Millipore), cruz Biotechnologies), pRb 4H1 (9309, Cell Signaling) and polymerase II, vol.3742, p.73
, Single (ChIP) and serial ChIP experiments were performed essentially as previously described. 49 The following primers were used: NOXA IS: 5 0 -CGTCTAGTTTCCCTACGTC-3 0, vol.5
, Statistical analysis of data was performed using one-tailed Student's t-test on GraphPad Prism
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