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, Increased arteriole density in the ischemic muscle of mice injected with TSA-treated ECFCs. Quantification of SMA + vessels 4 days after ECFCs transplantation. Results are expressed as the number of SMA + vessels per FOV ± SEM
, Immunostaining was performed on ischemic muscle cryosections with a human-specific mitochondrial Ab (hMito, green) and a murine-specific smooth muscle actin Ab (SMA, red) 4 days after injection of PBS (no cells), ECFCs pretreated with TSA (+TSA) or untreated ECFCs. Nuclei were stained with DAPI (blue). Left panels, scale bar: 50 mm
, TSA-treated ECFCs express higher levels of hCXCR4 in the ischemic muscle. qRT-PCR was performed on RNA isolated from whole dissected ischemic muscles injected with PBS or with ECFCs pretreated with TSA (+TSA) or not (ÀTSA) using primers specific for human CXCR4 (hCXCR4). qRT-PCR values for hCXCR4 are normalized to the total number of engrafted ECFCs per mouse muscle (quantified by measuring human Alu repeats as previously described
, Higher expression of hVEGFA in the ischemic muscle injected with TSA-treated ECFCs. Expression of human VEGFA (hVEGFA) was determined by qRT-PCR as described for hCXCR4 in (F)
, See also Figure S6
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