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, DCs received 50µg/ml LDL, or LDL-X or the equivalent amount of hGX-sPLA 2 brought by LDL-X in the cell culture (X 25, 10 or 5). When mentioned, the sPLA 2 inhibitor LY329722 (LY) was added 15 min before. (B-D) The mean fluorescence intensity for CD86 labeling was normalized to control iDCs receiving LDL buffer, 200 or 100nM hGX-sPLA 2 for 24h at RT (LDL-X), vol.500

, Functional DC maturation induced by LDL-X

, LDL-X was generated by treatment with 500nM hGX-sPLA 2 for 24h at RT. DCs received 50µg/ml LDL or LDL-X or 25nM hGX-sPLA 2 alone (X) or control buffer. DC supernatants were collected at day 6 and assayed for cytokines and chemokines secretion. Results represent the mean concentration±SE from 3 independent experiments. (B) LDL-X

, After a 24h-treatment with 50µg/ml LDL or LDL-X or buffer, DCs were washed and cocultured in triplicates for 5 days with allogeneic T cells. Mean IFN? secretion in MLR supernatants was normalized to control DCs for each experiment (fold increase). Results represent the mean±SE from 5 independent experiments

, LPC and fatty acids produced by sPLA 2 activity can modulate DC maturation

, B) 40µM LPC in the presence or absence of 50µg/ml LDL (C) 40µM LPC16:0 or LPC18:0 or LPC18:1. Their phenotype was analyzed at day 6 and CD86 expression is presented as fold induction compared to control iDCs. Results represent the mean±SE for, DCs were treated at day 5 for 24h with (A) 20 to 50µM LPC, vol.6

, DCs were treated at day 5 for 24h with 40µM fatty acid with or without 40µM LPC18:0 in the 25 presence of 50µg/ml LDL. DC phenotype was analyzed and CD86 expression is presented as fold induction compared to control iDCs