Skip to Main content Skip to Navigation
Journal articles

Endocytosis Switch Controlled by Transmembrane Osmotic Pressure and Phospholipid Number Asymmetry

Abstract : The dynamics of endocytosis in living K562 cells was investigated after the osmotic pressure of the external medium was decreased and the transmembrane phospholipid number asymmetry was increased. When the external pressure was decreased by a factor of 0.54, a sudden inhibition of endocytosis was observed. Under these conditions, the endocytosis suddenly recovered after the phospholipid number asymmetry was increased. The phospholipid asymmetry was generated by the addition of exogenous phosphatidylserine, which is translocated by the endogenous flippase activity to the inner layer of the membrane. The recovery of endocytosis is thus consistent with the view that the phospholipid number asymmetry can act as a budding force for endocytosis. Moreover, we quantitatively predict both the inhibition and recovery of endocytosis as first-order phase transitions, using a general model that assumes the existence of a transmembrane surface tension asymmetry as the budding driving force. In this model, the tension asymmetry is considered to be elastically generated by the activity of phospholipid pumping. We finally propose that cells may trigger genetic transcription responses after the internalization of cytokine-receptor complexes, which could be controlled by variations in the cytosolic or external pressure.
Complete list of metadatas

Cited literature [40 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02440878
Contributor : Emmanuel Farge <>
Submitted on : Wednesday, January 15, 2020 - 2:53:28 PM
Last modification on : Tuesday, July 21, 2020 - 3:58:52 AM
Long-term archiving on: : Thursday, April 16, 2020 - 3:40:27 PM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Citation

Cyril Rauch, Emmanuel Farge. Endocytosis Switch Controlled by Transmembrane Osmotic Pressure and Phospholipid Number Asymmetry. Biophysical Journal, Biophysical Society, 2000, 78 (6), pp.3036-3047. ⟨10.1016/S0006-3495(00)76842-1⟩. ⟨inserm-02440878⟩

Share

Metrics

Record views

73