Skip to Main content Skip to Navigation
Journal articles

Enhancement of endocytosis due to aminophospholipid transport across the plasma membrane of living cells

Abstract : Formation of intracellular vesicles is initiated by membrane budding. Here we test the hypothesis that the plasma membrane surface area asymmetry could be a driving force for vesicle formation during endocytosis. The inner layer phospholipid number was therefore increased by adding exogenous aminophospholipids to living cells, which were then translocated from the outer to the inner layer of the membrane by the ubiquitous flippase. Addition of either phosphatidylserine or phosphatidylethanolamine led to an enhancement of endocytosis, showing that the observed acceleration does not depend on the lipid polar head group. Conversely, a closely related aminophospholipid that is not recognized by the flippase, lyso-alpha-phosphatidylserine, inhibited endocytosis, and similar results were obtained with a cholesterol derivative that also remains in the plasma membrane outer layer. Thus an increase of lipid concentration in the inner layer enhanced internalization, whereas an increase of the lipid concentration in the outer layer inhibited internalization. These experiments suggest that transient asymmetries in lipid concentration might contribute to the formation of endocytic vesicles.
Complete list of metadatas

Cited literature [44 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02440860
Contributor : Emmanuel Farge <>
Submitted on : Wednesday, January 15, 2020 - 2:43:08 PM
Last modification on : Monday, March 16, 2020 - 8:58:20 AM
Document(s) archivé(s) le : Thursday, April 16, 2020 - 2:40:46 PM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Collections

Citation

Emmanuel Farge, David Ojcius, Agathe Subtil, Alice Dautry-Varsat. Enhancement of endocytosis due to aminophospholipid transport across the plasma membrane of living cells. American Journal of Physiology - Cell Physiology, American Physiological Society, 1999, 276 (3), pp.C725-C733. ⟨10.1152/ajpcell.1999.276.3.C725⟩. ⟨inserm-02440860⟩

Share

Metrics

Record views

81