 |
Journal articles
Endometrium as an early sensor of in vitro embryo manipulation technologies
Abstract : Implantation is crucial for placental development that will subsequently impact fetal growth and pregnancy success with consequences on postnatal health. We postulated that the pattern of genes expressed by the endometrium when the embryo becomes attached to the mother uterus could account for the final outcome of a pregnancy. As a model, we used the bovine species where the embryo becomes progressively and permanently attached to the endometrium from day 20 of gestation onwards. At that stage, we compared the endometrial genes profiles in the presence of an in vivo fertilized embryo (AI) with the endometrial patterns obtained in the presence of nuclear transfer (SCNT) or in vitro fertilized embryos (IVF), both displaying lower and different potentials for term development. Our data provide evidence that the endome-trium can be considered as a biological sensor able to fine-tune its physiology in response to the presence of embryos whose development will become altered much later after the implantation process. Compared with AI, numerous biological functions and several canonical pathways with a major impact on metabolism and immune function were found to be significantly altered in the endometrium of SCNT pregnancies at implantation, whereas the differences were less pronounced with IVF embryos. Determining the limits of the endometrial plasticity at the onset of implantation should bring new insights on the contribution of the maternal environment to the development of an embryo and the success of pregnancy. bovine ͉ implantation ͉ microarray ͉ nuclear transfer
Cited literature [41 references]
https://www.hal.inserm.fr/inserm-02440509
Contributor : Séverine Degrelle <>
Submitted on : Wednesday, January 15, 2020 - 11:16:13 AM
Last modification on : Thursday, August 5, 2021 - 3:52:24 AM
Long-term archiving on: : Thursday, April 16, 2020 - 1:59:40 PM
Contributor : Séverine Degrelle <>
Submitted on : Wednesday, January 15, 2020 - 11:16:13 AM
Last modification on : Thursday, August 5, 2021 - 3:52:24 AM
Long-term archiving on: : Thursday, April 16, 2020 - 1:59:40 PM
File
B2_Mansouri_PNAS_2009.pdf
Files produced by the author(s)