Skip to Main content Skip to Navigation
Journal articles

New Transcriptional Reporters to Quantify and Monitor PPAR γ Activity

Abstract : The peroxisome-proliferator-activated-receptor-(PPAR) is a member of the nuclear receptor superfamily that plays a critical role in diverse biological processes, including adipogenesis, lipid metabolism, and placental development. To study the activity of PPAR , we constructed two new reporter genes: a fluorescent GFP-tagged histone-2B (PPRE-H2B-eGFP) and a secreted nanoluciferase (PPRE-pNL1.3[secNluc]). This study demonstrates their usage to monitor PPAR activity in different cell types and screen for PPAR 's potential ligands.
Complete list of metadatas

Cited literature [27 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02440451
Contributor : Séverine Degrelle <>
Submitted on : Wednesday, January 15, 2020 - 10:57:15 AM
Last modification on : Friday, March 27, 2020 - 2:49:04 AM

File

E6_Degrelle_PPARResearch_2017....
Publication funded by an institution

Identifiers

Citation

Severine Degrelle, Hussein Shoaito, Thierry Fournier. New Transcriptional Reporters to Quantify and Monitor PPAR γ Activity. PPAR Research, Hindawi Publishing Corporation, 2017, 2017, pp.1-7. ⟨10.1155/2017/6139107⟩. ⟨inserm-02440451⟩

Share

Metrics

Record views

78

Files downloads

190