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Transcriptome analysis of primary bovine extra-embryonic cultured cells

Abstract : The dataset described in this article pertains to the article by Hue et al. (2015) entitled "Primary bovine extra-embryonic cultured cells: A new resource for the study of in vivo peri-implanting phenotypes and mesoderm formation" [1]. In mammals, extra-embryonic tissues are essential to support not only embryo patterning but also embryo survival, especially in late implanting species. These tissues are composed of three cell types: trophoblast (bTCs), endoderm (bXECs) and mesoderm (bXMCs). Until now, it is unclear how these cells interact. In this study, we have established primary cell cultures of extra-embryonic tissues from bovine embryos collected at day-18 after artificial insemination. We used our homemade bovine 10K array (GPL7417) to analyze the gene expression profiles of these primary extra-embryonic cultured cells compared to the corresponding cells from in vivo micro-dissected embryos. Here, we described the experimental design, the isolation of bovine extra-embryonic cell types as well as the microarray expression analysis. The dataset has been deposited in Gene Expression Omnibus (GEO) (accession number GSE52967). Finally, these primary cell cultures were a powerful tool to start studying their cellular properties, and will further allow in vitro studies on cellular interactions among extra-embryonic tissues, and potentially between extra-embryonic vs embryonic tissues.
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https://www.hal.inserm.fr/inserm-02440415
Contributor : Séverine Degrelle <>
Submitted on : Wednesday, January 15, 2020 - 10:48:29 AM
Last modification on : Thursday, July 9, 2020 - 9:17:45 AM
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Severine Degrelle. Transcriptome analysis of primary bovine extra-embryonic cultured cells. Genomics Data, Elsevier, 2015, 6, pp.110-111. ⟨10.1016/j.gdata.2015.08.019⟩. ⟨inserm-02440415⟩

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