 |
Journal articles
A new subtype of bone sarcoma defined by BCOR-CCNB3 gene fusion
Abstract : The identification of subtype-specific translocations has revolutionized the diagnostics of sarcoma and has provided new insight into oncogenesis. We used RNA-seq to investigate samples from individuals diagnosed with small round cell tumors of bone, possibly Ewing sarcoma, but which lacked the canonical EWSR1-ETS translocation. A new fusion was observed between BCOR (encoding the BCL6 co-repressor) and CCNB3 (encoding the testis-specific cyclin B3) on the X chromosome. RNA-seq results were confirmed by RT-PCR and through cloning of the tumor-specific genomic translocation breakpoints. In total, 24 BCOR-CCNB3-positive tumors were identified among a series of 594 sarcoma cases. Gene profiling experiments indicated that BCOR-CCNB3-positive cases are biologically distinct from other sarcomas, particularly Ewing sarcoma. Finally, we show that CCNB3 immunohistochemistry is a powerful diagnostic marker for this subgroup of sarcoma and that overexpression of BCOR-CCNB3 or of truncated CCNB3 activates S phase in NIH3T3 cells. Thus, the intrachromosomal X-chromosome fusion described here represents a new subtype of bone sarcoma caused by a newly identified gene fusion mechanism.
Cited literature [19 references]
https://www.hal.inserm.fr/inserm-02440379
Contributor : Franck Tirode <>
Submitted on : Wednesday, January 15, 2020 - 10:37:27 AM
Last modification on : Monday, July 27, 2020 - 5:12:11 PM
Long-term archiving on: : Thursday, April 16, 2020 - 2:15:47 PM
Contributor : Franck Tirode <>
Submitted on : Wednesday, January 15, 2020 - 10:37:27 AM
Last modification on : Monday, July 27, 2020 - 5:12:11 PM
Long-term archiving on: : Thursday, April 16, 2020 - 2:15:47 PM
File
BCOR.NatGenet.Post.Editor.vers...
Files produced by the author(s)