 |
Journal articles
Systemic Gene Delivery by Single-Dose Intracardiac Administration of scAAV2/9 and scAAV2/rh10 Variants in Newborn Rats
Abstract : Abstract
Recombinant adeno-associated virus serotype 9 (rAAV2/9) and pseudotype rhesus-10 (rAAV2/rh10) are used for gene delivery, especially into the central nervous system. Both serotypes cross the blood-brain barrier and mediate stable long-term transduction in dividing and nondividing cells. Among possible routes of administration, intracardiac injection holds the potential for widespread vector diffusion associated with a relatively simple approach. In this study adopting the intracardiac route, we compare the cell-specific tropism and transfection efficacy of a panel of engineered rAAV2/9 and rAAV2/rh10 vectors encoding the enhanced green fluorescent protein. We observed transduction in the brain and peripherally, with a predominant neuronal tropism while the various serotypes achieved different expression patterns.
https://www.hal.inserm.fr/inserm-02439491
Contributor : Benjamin Dehay <>
Submitted on : Tuesday, January 14, 2020 - 4:01:05 PM
Last modification on : Thursday, November 12, 2020 - 9:30:16 AM
Contributor : Benjamin Dehay <>
Submitted on : Tuesday, January 14, 2020 - 4:01:05 PM
Last modification on : Thursday, November 12, 2020 - 9:30:16 AM