,
, Merck) solution: 20 mg X-Gal/ml of N,N-Dimethylformamide (DMF; Sigma)
100 ml Z-Buffer; 0.27 ml ?-Mercaptoethanol; 1.67 ml X-Gal solution. 2.5.2. Liquid culture assay ,
100 ml Z-buffer; 0.27 ml ?-Mercaptoethanol ,
solution: dissolve 4 mg ONPG (o-nitrophenyl-?-D-galactopyranoside ,
, Sigma)/ ml Z-buffer (prepare ca. 1 hour before each use)
, 10x S-buffer (for 500 ml): 68 g KH 2 PO 4, vol.9
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