Skip to Main content Skip to Navigation
Journal articles

A multiepitope peptide vaccine against HCV stimulates neutralizing humoral and persistent cellular responses in mice

Abstract : BACKGROUND: Although DAAs hold promise to significantly reduce rates of chronic HCV infections, its eradication still requires development of an effective vaccine. Prolonged T cell responses and cross neutralizing antibodies are ideal for vaccination against the infection. We aimed to design and synthesize a 6 multi epitope peptide vaccine candidate and provide evidence for production of extended cellular and neutralizing Abs in mice. METHODS: Six peptides derived from conserved epitopes in E1, E2 (n = 2),NS4B, NS5A and NS5B were designed, synthesized in a multiple antigenic peptide (MAP) form and administered w/o adjuvant to BALB/c mice as HCVp6-MAP at doses ranging from 800 ng to 16 μg. Humoral responses to structural epitopes were assayed by ELISA at different times after injection. ELISpot assay was used to evaluate IFN ɣ producing CD4+/ CD8+ T- lymphocytes at extended durations i.e. > 20 weeks. Viral neutralization by mice sera was tested for genotypes 2a (JFH1) and a chimeric 2a/4a virus (ED43/JFH1) in HCVcc culture. RESULTS: HCVp6-MAP confers potent viral neutralization and specific cellular responses at > 1600 ng/ animal for at least 20 weeks. CONCLUSION: We report on a promising anti HCV vaccine for future studies on permissive hosts and in clinical trials.
Complete list of metadatas

Cited literature [80 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02438162
Contributor : Myriam Bodescot <>
Submitted on : Tuesday, January 14, 2020 - 10:13:09 AM
Last modification on : Wednesday, September 30, 2020 - 4:28:03 PM
Long-term archiving on: : Wednesday, April 15, 2020 - 2:32:37 PM

File

s12879-019-4571-5.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Reham Dawood, Rehab Moustafa, Tawfeek Abdelhafez, Reem El-Shenawy, Yasmine El-Abd, et al.. A multiepitope peptide vaccine against HCV stimulates neutralizing humoral and persistent cellular responses in mice. BMC Infectious Diseases, BioMed Central, 2019, 19 (1), pp.932. ⟨10.1186/s12879-019-4571-5⟩. ⟨inserm-02438162⟩

Share

Metrics

Record views

122

Files downloads

242