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A Mechanism for Translationally Coupled mRNA Turnover: Interaction between the Poly(A) Tail and a c-fos RNA Coding Determinant via a Protein Complex

Abstract : mRNA turnover mediated by the major protein-coding-region determinant of instability (mCRD) of the c-fos proto-oncogene transcript illustrates a functional interplay between mRNA turnover and translation. We show that the function of mCRD depends on its distance from the poly(A) tail. Five mCRD-associated proteins were identified: Unr, a purine-rich RNA binding protein; PABP, a poly(A) binding protein; PAIP-1, a poly(A) binding protein interacting protein; hnRNP D, an AU-rich element binding protein; and NSAP1, an hnRNP R-like protein. These proteins form a multiprotein complex. Overexpression of these proteins stabilized mCRD-containing mRNA by impeding deadenylation. We propose that a bridging complex forms between the poly(A) tail and the mCRD and ribosome transit disrupts or reorganizes the complex, leading to rapid RNA deadenylation and decay.
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Christophe Grosset, Chyi-Ying Chen, Nianhua Xu, Nahum Sonenberg, Hélène Jacquemin-Sablon, et al.. A Mechanism for Translationally Coupled mRNA Turnover: Interaction between the Poly(A) Tail and a c-fos RNA Coding Determinant via a Protein Complex. Cell, Elsevier, 2000, 103, pp.29 - 40. ⟨10.1016/s0092-8674(00)00102-1⟩. ⟨inserm-02437952⟩

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