, ) software by using nonparametric Mann-Whitney unpaired tests. Statistical analyses involving more than two conditions were performed with nonparametric Kruskal-Wallis unpaired tests followed by post-tests or Friedman test, Data are reported as means ± standard deviation (SD)

P. Bousso and H. D. Moreau, Functional immunoimaging: the revolution continues, Nat. Rev. Immunol. 2012, vol.12, pp.858-864

S. Celli, M. L. Albert, and P. Bousso, Visualizing the innate and adaptive immune responses underlying allograft rejection by two-photon microscopy, Nat. Med, vol.17, pp.744-749, 2011.
URL : https://hal.archives-ouvertes.fr/pasteur-01402079

R. N. Germain, E. A. Robey, and M. D. Cahalan, A decade of imaging cellular motility and interaction dynamics in the immune system, Science, vol.336, pp.1676-1681, 2012.

S. Stoll, J. Delon, T. M. Brotz, and R. N. Germain, Dynamic imaging of T cell-dendritic cell interactions in lymph nodes, Science, vol.296, pp.1873-1876, 2002.

A. Abtin, R. Jain, A. J. Mitchell, B. Roediger, A. J. Brzoska et al., Perivascular macrophages mediate neutrophil recruitment during bacterial skin infection, Nat. Immunol, vol.15, pp.45-53, 2014.

J. Tang, N. Van-panhuys, W. Kastenmuller, and R. N. Germain, The future of immunoimaging-deeper, bigger, more precise, and definitively more colorful, Eur. J. Immunol, vol.43, pp.1407-1412, 2013.

M. Del-pilar-martin, W. C. Weldon, V. G. Zarnitsyn, D. G. Koutsonanos, H. Akbari et al., Local response to microneedlebased influenza immunization in the skin, MBio, vol.3, pp.12-00012, 2012.

T. Rattanapak, J. C. Birchall, K. Young, A. Kubo, S. Fujimori et al., Dynamic visualization of dendritic cell-antigen interactions in the skin following transcutaneous immunization, PLoS One, vol.9, p.89503, 2014.

R. N. Germain, Vaccines and the future of human immunology, Immunity, vol.33, pp.441-450, 2010.

B. Pulendran and R. Ahmed, Immunological mechanisms of vaccination, Nat. Immunol. 2011, vol.12, pp.509-517

M. M. Davis, A prescription for human immunology, Immunity, vol.29, pp.835-838, 2008.

R. L. Coffman and E. M. Hessel, Nonhuman primate models of asthma

, J. Exp. Med, vol.201, pp.1875-1879, 2005.

J. Seok, H. S. Warren, A. G. Cuenca, M. N. Mindrinos, H. V. Baker et al., Genomic responses in mouse models poorly mimic human inflammatory diseases, Proc. Natl. Acad. Sci, vol.110, pp.3507-3512, 2013.

H. B. Van-der-worp, D. W. Howells, E. S. Sena, M. J. Porritt, S. Rewell et al., Can animal models of disease reliably inform human studies, PLoS Med, vol.7, p.1000245, 2010.

K. Shortman, M. H. Lahoud, and I. Caminschi, Improving vaccines by targeting antigens to dendritic cells, Exp. Mol. Med, vol.41, pp.61-66, 2009.

J. L. Heeney, Preclinical primate studies of HIV-1-envelope-based vaccines: towards human clinical trials, Curr. Opin. HIV AIDS, vol.1, pp.336-343, 2006.

B. S. Graham, Advances in antiviral vaccine development, Immunol. Rev, vol.255, pp.230-242, 2013.

S. M. Kaech, E. J. Wherry, and R. Ahmed, Effector and memory T-cell differentiation: implications for vaccine development, Nat. Rev. Immunol, vol.2, pp.251-262, 2002.

F. O. Nestle, P. Di-meglio, J. Z. Qin, and B. J. Nickoloff, Skin immune sentinels in health and disease, Nat. Rev. Immunol, vol.9, pp.679-691, 2009.

C. Liard, S. Munier, M. Arias, A. Joulin-giet, O. Bonduelle et al., Targeting of HIV-p24 particle-based vaccine into differential skin layers induces distinct arms of the immune responses, Vaccine, vol.29, pp.6379-6391, 2011.

A. L. Flamar, S. Zurawski, F. Scholz, I. Gayet, L. Ni et al., Noncovalent assembly of anti-dendritic cell antibodies and antigens for evoking immune responses in vitro and in vivo

, J. Immunol, vol.189, pp.2645-2655, 2012.

B. J. Flynn, K. Kastenmuller, U. Wille-reece, G. D. Tomaras, M. Alam et al., Immunization with HIV Gag targeted to dendritic cells followed by recombinant New York vaccinia virus induces robust T-cell immunity in nonhuman primates, Proc. Natl

. Acad, . Sci, and . Usa, , vol.108, pp.7131-7136, 2011.

P. J. Tacken, I. J. De-vries, R. Torensma, and C. G. Figdor, Dendriticcell immunotherapy: from ex vivo loading to in vivo targeting, Nat. Rev. Immunol, vol.7, pp.790-802, 2007.

E. Klechevsky, A. L. Flamar, Y. Cao, J. P. Blanck, M. Liu et al., Cross-priming CD8+ T cells by targeting antigens to human dendritic cells through DCIR, Blood, vol.116, pp.1685-1697, 2010.

D. Li, G. Romain, A. L. Flamar, D. Duluc, M. Dullaers et al., Targeting self-and foreign antigens to dendritic cells via DC-ASGPR generates IL-10-producing suppressive CD4+ T cells, J. Exp. Med. 2012, vol.209, pp.109-121

J. Li, F. Ahmet, L. C. Sullivan, A. G. Brooks, S. J. Kent et al., Antibodies targeting Clec9A promote strong humoral immunity without adjuvant in mice and non-human primates, Eur. J. Immunol, vol.45, pp.854-864, 2015.

C. Trumpfheller, M. P. Longhi, M. Caskey, J. Idoyaga, L. Bozzacco et al., Dendritic cell-targeted protein vaccines: a novel approach to induce T-cell immunity, J. Intern. Med, vol.271, pp.183-192, 2012.

R. Ma, J. L. Du, J. Huang, and C. Y. Wu, Additive effects of CpG ODN and R-848 as adjuvants on augmenting immune responses to HBsAg vaccination, Biochem. Biophys. Res. Commun, vol.361, pp.537-542, 2007.

K. Matthews, N. P. Chung, P. J. Klasse, J. P. Moore, and R. W. Sanders, Potent induction of antibody-secreting B cells by human dermal-derived CD14+ dendritic cells triggered by dual TLR ligation, J. Immunol, vol.189, pp.5729-5744, 2012.

Y. Torii, T. Ito, R. Amakawa, H. Sugimoto, H. Amuro et al., Imidazoquinoline acts as immune adjuvant for functional alteration of thymic stromal lymphopoietin-mediated allergic T cell response, J. Immunol, vol.181, pp.5340-5349, 2008.

M. Kwissa, H. I. Nakaya, H. Oluoch, and B. Pulendran, Distinct TLR adjuvants differentially stimulate systemic and local innate immune responses in nonhuman primates, Blood, vol.119, pp.2044-2055, 2012.

V. Flacher, C. H. Tripp, P. Stoitzner, B. Haid, S. Ebner et al., Epidermal Langerhans cells rapidly capture and present antigens from C-type lectin-targeting antibodies

, J. Invest. Dermatol, vol.130, pp.755-762, 2010.

P. Stoitzner, S. Schaffenrath, C. H. Tripp, D. Reider, K. Komenda et al., Human skin dendritic cells can be targeted in situ by intradermal injection of antibodies against lectin receptors, Exp. Dermatol, vol.23, pp.909-918, 2014.

M. V. Dhodapkar, M. Sznol, B. Zhao, D. Wang, R. D. Carvajal et al., Induction of antigen-specific immunity with a vaccine targeting NY-ESO-1 to the dendritic cell receptor DEC-205, Sci. Transl. Med, vol.6, pp.232-251, 2014.

R. L. Sabado, A. Pavlick, S. Gnjatic, C. M. Cruz, I. Vengco et al., Resiquimod as an immunologic adjuvant for NY-ESO-1 protein vaccination in patients with high-risk melanoma, Cancer Immunol. Res, vol.3, pp.278-287, 2015.

C. L. Ahonen, S. J. Gibson, R. M. Smith, L. K. Pederson, J. M. Lindh et al., Dendritic cell maturation and subsequent enhanced T-cell stimulation induced with the novel synthetic immune response modifier R-848, Cell Immunol, vol.197, pp.62-72, 1999.

T. L. Wagner, C. L. Ahonen, A. M. Couture, S. J. Gibson, R. L. Miller et al., Modulation of TH1 and TH2 cytokine production with the immune response modifiers, R-848 and imiquimod, Cell Immunol, vol.191, pp.10-19, 1999.

M. A. Tomai and J. P. Vasilakos, TLR-7 and -8 agonists as vaccine adjuvants, Expert Rev. Vaccines, vol.10, pp.405-407, 2011.

J. Tel, S. P. Sittig, R. A. Blom, L. J. Cruz, G. Schreibelt et al., Targeting uptake receptors on human plasmacytoid dendritic cells triggers antigen cross-presentation and robust type I IFN secretion, J. Immunol, vol.191, pp.5005-5012, 2013.

O. Epaulard, L. Adam, C. Poux, G. Zurawski, N. Salabert et al., Macrophage-and neutrophil-derived TNF-alpha instructs skin langerhans cells to prime antiviral immune responses, J. Immunol, vol.193, pp.2416-2426, 2014.

T. Meyer, C. Surber, L. E. French, and E. Stockfleth, Resiquimod, a topical drug for viral skin lesions and skin cancer, Expert Opin. Investig. Drugs, vol.22, pp.149-159, 2013.

C. Iking-konert, B. Ostendorf, O. Sander, M. Jost, C. Wagner et al., Transdifferentiation of polymorphonuclear neutrophils to dendritic-like cells at the site of inflammation in rheumatoid arthritis: evidence for activation by T cells, Ann. Rheum. Dis, vol.64, pp.1436-1442, 2005.

L. Adam, P. Rosenbaum, A. Cosma, R. Le-grand, and F. Martinon, Identification of skin immune cells in non-human primates, J. Immunol. Methods, vol.426, pp.42-49, 2015.
URL : https://hal.archives-ouvertes.fr/inserm-02437893

C. Auffray, D. Fogg, M. Garfa, G. Elain, O. Join-lambert et al., Monitoring of blood vessels and tissues by a population of monocytes with patrolling behavior, Science, vol.317, pp.666-670, 2007.
URL : https://hal.archives-ouvertes.fr/pasteur-00337698

F. Geissmann, M. G. Manz, S. Jung, M. H. Sieweke, M. Merad et al., Development of monocytes, macrophages, and dendritic cells, Science, vol.327, pp.656-661, 2010.
URL : https://hal.archives-ouvertes.fr/hal-00502972

J. Idoyaga, C. Cheong, K. Suda, N. Suda, J. Y. Kim et al., Cutting edge: langerin/CD207 receptor on dendritic cells mediates efficient antigen presentation on MHC I and II products in vivo, J. Immunol, vol.180, pp.3647-3650, 2008.

J. Idoyaga, A. Lubkin, C. Fiorese, M. H. Lahoud, I. Caminschi et al., Comparable T helper 1 (Th1) and CD8 T-cell immunity by targeting HIV gag p24 to CD8 dendritic cells within antibodies to

D. Langerin and C. , Proc. Natl. Acad. Sci, vol.108, pp.2384-2389, 2011.

E. Klechevsky, R. Morita, M. Liu, Y. Cao, S. Coquery et al., Functional specializations of human epidermal Langerhans cells and CD14+ dermal dendritic cells, Immunity, vol.29, pp.497-510, 2008.

T. Ouchi, A. Kubo, M. Yokouchi, T. Adachi, T. Kobayashi et al., Langerhans cell antigen capture through tight junctions confers preemptive immunity in experimental staphylococcal scalded skin syndrome, J. Exp. Med, vol.208, pp.2607-2613, 2011.

M. Jurk, F. Heil, J. Vollmer, C. Schetter, A. M. Krieg et al., Human TLR7 or TLR8 independently confer responsiveness to the antiviral compound R-848, Nat. Immunol, vol.3, p.499, 2002.

M. P. Radsak, H. R. Salih, H. G. Rammensee, and H. Schild, Triggering receptor expressed on myeloid cells-1 in neutrophil inflammatory responses: differential regulation of activation and survival, J. Immunol, vol.172, pp.4956-4963, 2004.

M. P. Reddy, C. A. Kinney, M. A. Chaikin, A. Payne, J. Fishman-lobell et al., Elimination of Fc receptor-dependent effector functions of a modified IgG4 monoclonal antibody to human CD4, J. Immunol, vol.164, pp.1925-1933, 2000.

B. Z. Igyarto, K. Haley, D. Ortner, A. Bobr, M. Gerami-nejad et al., Skin-resident murine dendritic cell subsets promote distinct and opposing antigen-specific T helper cell responses
URL : https://hal.archives-ouvertes.fr/hal-00667766