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Optimization of a fragment linking hit toward Dengue and Zika virus NS5 methyltransferases inhibitors

Abstract : No antiviral drugs to treat or prevent life-threatening flavivirus infections such as those caused by mosquito-borne Dengue (DENV) and more recently Zika (ZIKV) viruses are yet available. We aim to develop, through a structure-based drug design approach, novel inhibitors targeting the NS5 AdoMet-dependent mRNA methyltransferase (MTase), a viral protein involved in the RNA capping process essential for flaviviruses replication. Herein, we describe the optimization of a hit (5) identified using fragment-based and structure-guided linking techniques, which binds to a proximal site of the AdoMet binding pocket. X-ray crystallographic structures and computational docking were used to guide our optimization process and lead to compounds 30 and 33 (DENV IC50 = 26 μM and 23 μM; ZIKV IC50 = 28 μM and 19 μM, respectively), two representatives of novel non-nucleoside inhibitors of flavivirus MTases.
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https://www.hal.inserm.fr/inserm-02416983
Contributor : Bruno Coutard <>
Submitted on : Tuesday, December 17, 2019 - 10:21:50 PM
Last modification on : Monday, July 6, 2020 - 1:28:03 PM

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Jessica Hernandez, Laurent Hoffer, Bruno Coutard, Gilles Querat, Philippe Roche, et al.. Optimization of a fragment linking hit toward Dengue and Zika virus NS5 methyltransferases inhibitors. European Journal of Medicinal Chemistry, Elsevier, 2019, 161, pp.323-333. ⟨10.1016/j.ejmech.2018.09.056⟩. ⟨inserm-02416983⟩

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