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Structure and oligomerization state of the C-terminal region of the Middle East respiratory syndrome coronavirus nucleoprotein

Abstract : Middle East respiratory syndrome coronavirus (MERS-CoV) is a human pathogen responsible for a severe respiratory illness that emerged in 2012. Structural information about the proteins that constitute the viral particle is scarce. In order to contribute to a better understanding of the nucleoprotein (N) in charge of RNA genome encapsidation, the structure of the C-terminal domain of N from MERS-CoV obtained using single-crystal X-ray diffraction is reported here at 1.97 Å resolution. The molecule is present as a dimer in the crystal structure and this oligomerization state is confirmed in solution, as measured by additional methods including small-angle X-ray scattering measurements. Comparisons with the structures of the C-terminal domains of N from other coronaviruses reveals a high degree of structural conservation despite low sequence conservation, and differences in electrostatic potential at the surface of the protein.
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Submitted on : Tuesday, December 17, 2019 - 10:16:52 PM
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Thi Hong Van Nguyen, Julie Lichiere, Bruno Canard, Nicolas Papageorgiou, Sarah Attoumani, et al.. Structure and oligomerization state of the C-terminal region of the Middle East respiratory syndrome coronavirus nucleoprotein. Acta crystallographica. Section D, Structural biology, International Union of Crystallography, 2019, 75 (1), pp.8-15. ⟨10.1107/S2059798318014948⟩. ⟨inserm-02416977⟩

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