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Conformational plasticity of the VEEV macro domain is important for binding of ADP-ribose

Abstract : Venezuelan equine encephalitis virus (VEEV) is a new world alphavirus which can be involved in several central nervous system disorders such as encephalitis and meningitis. The VEEV genome codes for 4 non-structural proteins (nsP), of which nsP3 contains a Macro domain. Macro domains (MD) can be found as stand-alone proteins or embedded within larger proteins in viruses, bacteria and eukaryotes. Their most common feature is the binding of ADP-ribose (ADPr), while several macro domains act as ribosylation writers, erasers or readers. Alphavirus MD erase ribosylation but their precise contribution in viral replication is still under investigation. NMR-driven titration experiments of ADPr in solution with the VEEV macro domain (in apo- and complex state) show that it adopts a suitable conformation for ADPr binding. Specific experiments indicate that the flexibility of the loops β5-α3 and α3-β6 is critical for formation of the complex and assists a wrapping mechanism for ADPr binding. Furthermore, along with this sequence of events, the VEEV MD undergoes a conformational exchange process between the apo state and a low-populated "dark" conformational state.
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Submitted on : Friday, October 22, 2021 - 5:28:19 PM
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Garyfallia Makrynitsa, Dioni Ntonti, Konstantinos Marousis, Maria Birkou, Minos-Timotheos Matsoukas, et al.. Conformational plasticity of the VEEV macro domain is important for binding of ADP-ribose. Journal of Structural Biology, Elsevier, 2019, 206 (1), pp.119-127. ⟨10.1016/j.jsb.2019.02.008⟩. ⟨inserm-02416973⟩



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