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Journal articles
CENP-A nucleosome clusters form rosette-like structures around HJURP during G1
Abstract : CENP-A is an essential histone H3 variant that epigenetically marks the centromeric region of chromosomes. Here we show that CENP-A nucleosomes form characteristic clusters during the G1 phase of the cell cycle. 2D and 3D super-resolution microscopy and segmentation analysis reveal that these clusters encompass a globular rosette-like structure, which evolves into a more compact structure in late G1. The rosette-like clusters contain numerous CENP-A molecules and form a large cellular structure of ∼250-300 nm diameter with remarkably similar shapes for each centromere. Co-localization analysis shows that HJURP, the CENP-A chaperone, is located in the center of the rosette and serves as a nucleation point. The discovery of an HJURP-mediated CENP-A nucleation in human cells and its structural description provide important insights into the mechanism of CENP-A deposition and the organization of CENP-A chromatin in the centromeric region.
Cited literature [52 references]
https://www.hal.inserm.fr/inserm-02409790
Contributor : Bruno Klaholz Connect in order to contact the contributor
Submitted on : Friday, December 13, 2019 - 3:33:43 PM
Last modification on : Wednesday, November 3, 2021 - 5:03:41 AM
Contributor : Bruno Klaholz Connect in order to contact the contributor
Submitted on : Friday, December 13, 2019 - 3:33:43 PM
Last modification on : Wednesday, November 3, 2021 - 5:03:41 AM