Skip to Main content Skip to Navigation
Journal articles

N6-Methyladenosine methyltransferase ZCCHC4 mediates ribosomal RNA methylation

Abstract : N6-Methyladenosine (m6A) RNA modification is present in messenger RNAs (mRNA), ribosomal RNAs (rRNA), and spliceosomal RNAs (snRNA) in humans. Although mRNA m6A modifications have been extensively studied and shown to play critical roles in many cellular processes, the identity of m6A methyltransferases for rRNAs and the function of rRNA m6A modifications are unknown. Here we report a new m6A methyltransferase, ZCCHC4, which primarily methylates human 28S rRNA and also interacts with a subset of mRNAs. ZCCHC4 knockout eliminates m6A4220 modification in 28S rRNA, reduces global translation, and inhibits cell proliferation. We also find that ZCCHC4 protein is overexpressed in hepatocellular carcinoma tumors, and ZCCHC4 knockout significantly reduces tumor size in a xenograft mouse model. Our results highlight the functional significance of an rRNA m6A modification in translation and in tumor biology.
Complete list of metadatas

https://www.hal.inserm.fr/inserm-02409786
Contributor : Bruno Klaholz <>
Submitted on : Friday, December 13, 2019 - 3:32:54 PM
Last modification on : Friday, January 10, 2020 - 9:10:22 PM

Links full text

Identifiers

Collections

Citation

Honghui Ma, Xiaoyun Wang, Jiabin Cai, Qing Dai, S. Kundhavai Natchiar, et al.. N6-Methyladenosine methyltransferase ZCCHC4 mediates ribosomal RNA methylation. Nature Chemical Biology, Nature Publishing Group, 2019, 15 (1), pp.88-94. ⟨10.1038/s41589-018-0184-3⟩. ⟨inserm-02409786⟩

Share

Metrics

Record views

58