, eukaryotic mRNA without cap-structure and poly(A)-tail: a cryo electron tomography study, Nucleic Acids Res, vol.42, pp.9461-9469
Toward automated crystallographic structure refinement with phenix.refine, Acta Cryst, vol.68, pp.352-367, 2012. ,
Atomic model of the F420-reducing [NiFe] hydrogenase by electron cryo-microscopy using a direct electron detector, Elife, vol.3, p.1963, 2014. ,
SharpViSu: integrated analysis and segmentation of super-resolution microscopy data, Bioinformatics, vol.32, pp.2239-2241, 2016. ,
ClusterViSu, a method for clustering of protein complexes by Voronoi tessellation in super-resolution microscopy, Sci. Rep, vol.6, p.24084, 2016. ,
Site-Specific Cryo-focused Ion Beam Sample Preparation Guided by 3D Correlative Microscopy, Biophys. J, vol.110, pp.860-869, 2016. ,
, Situ Cryo-Electron Tomography: A Post-Reductionist Approach to Structural Biology, vol.428, pp.332-343, 2016.
Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles, vol.2, p.461, 2013. ,
Discrete structure of an RNA folding intermediate revealed by cryo-electron microscopy, J. Am. Chem. Soc, vol.132, pp.16352-163523, 2010. ,
) 2.3Å resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition, Science, vol.351, pp.871-875, 2016. ,
EMRinger: side chain-directed model and map validation for 3D cryo-electron microscopy, Nat. Methods, vol.12, pp.943-946, 2015. ,
Nuclear pore complex structure and dynamics revealed by cryoelectron tomography, Science, vol.306, pp.1387-1390, 2004. ,
IBiSS, a versatile and interactive tool for integrated sequence and 3D structure analysis of large macromolecular complexes, Bioinformatics, vol.31, pp.3339-3344, 2015. ,
Crystal structure of the eukaryotic ribosome, Science, vol.330, pp.1203-1209, 2010. ,
The three-dimensional organization of polyribosomes in intact human cells, Mol. Cell, vol.39, pp.560-569, 2010. ,
Structural biology in situ -the potential of subtomogram averaging, Curr. Opin. Struct. Biol, vol.23, pp.261-267, 2013. ,
Beam-induced motion of vitrified specimen on holey carbon film, J. Struct. Biol, vol.177, pp.630-637, 2012. ,
The PILATUS 1M detector, J. Synchrotron Radiat, vol.13, pp.120-130, 2006. ,
Tools for macromolecular model building and refinement into electron cryo-microscopy reconstructions, Acta Crystallogr. D Biol. Crystallogr, vol.71, pp.136-153, 2015. ,
Movies of ice-embedded particles enhance resolution in electron cryo-microscopy, Structure, vol.20, pp.1823-1828, 2012. ,
Three-dimensional reconstruction methods in Single Particle Analysis from transmission electron microscopy data, Arch. Biochem. Biophys, vol.581, pp.39-48, 2015. ,
Cryo electron microscopy to determine the structure of macromolecular complexes, Methods, vol.95, pp.78-85, 2016. ,
EIGER detector: application in macromolecular crystallography, Acta Crystallogr. D Struct. Biol, vol.72, pp.1036-1048, 2016. ,
Dynamo Catalogue: Geometrical tools and data management for particle picking in subtomogram averaging of cryo-electron tomograms, J. Struct. Biol, vol.16, pp.30111-30113, 2016. ,
Architecture of the type IVa pilus machine, Science, vol.351, p.2001, 2016. ,
Structural dynamics of ribosome subunit association studied by mixing-spraying time-resolved cryogenic electron microscopy, Structure, vol.23, pp.1097-1105, 2015. ,
Molecular interactions in rotavirus assembly and uncoating seen by high-resolution cryo-EM, Proc. Natl. Acad. Sci. U.S.A, vol.106, pp.10644-10648, 2009. ,
Autofocused 3D classification of cryoelectron subtomograms, Structure, vol.22, pp.1528-1537, 2014. ,
) 3.9Å structure of the nucleosome core particle determined by phase-plate cryo-EM, Nucleic Acids Res, vol.44, pp.8013-8019, 2016. ,
Zernike phase-contrast electron cryotomography applied to marine cyanobacteria infected with cyanophages, Nat. Protoc, vol.9, pp.2630-2642, 2014. ,
Cryo-EM single particle analysis with the Volta phase plate, Elife, vol.5, p.13046, 2016. ,
Volta potential phase plate for in-focus phase contrast transmission electron microscopy, Proc. Natl Acad. Sci. U.S.A, vol.44, pp.15635-15640, 2014. ,
Cryo-electron microscopy of vitrified specimens, Q. Rev. Biophys, vol.21, pp.129-228, 1988. ,
Developments in cryo-electron tomography for in situ structural analysis, Arch. Biochem. Biophys, vol.581, pp.78-85, 2015. ,
Imaging of organelles by electron microscopy reveals protein-protein interactions in mitochondria and chloroplasts, FEBS Lett, vol.584, pp.2510-2515, 2010. ,
Cryo-EM of viruses and vaccine design, Proc. Nat. Acad. Sci. U.S.A, vol.113, pp.8903-8905, 2016. ,
IF2 Initiation factor 2 crystal structure reveals a different domain organization from eukaryotic initiation factor 5B and mechanism among translational GTPases, Proc. Nat. Acad. Sci. U.S.A, vol.110, pp.15662-15667, 2013. ,
Features and development of Coot, Acta Crystallogr. D Biol. Crystallogr, vol.66, pp.486-501, 2010. ,
Ribosome dynamics and tRNA movement by time-resolved electron cryomicroscopy, Nature, vol.466, pp.329-333, 2010. ,
Structure of the E. coli ribosome-EF-Tu complex at <3Å resolution by Cs-corrected cryo-EM, Nature, vol.520, pp.567-570, 2015. ,
Identification of macromolecular complexes in cryoelectron tomograms of phantom cells, Proc. Natl. Acad. Sci. U.S.A, vol.99, pp.14153-14158, 2002. ,
Computational separation of conformational heterogeneity using cryo-electron tomography and 3D sub-volume averaging, J. Struct. Biol, vol.178, pp.165-176, 2012. ,
In-focus electrostatic Zach phase plate imaging for transmission electron microscopy with tunable phase contrast of frozen hydrated biological samples, Microsc. Microanal, vol.1, pp.175-183, 2014. ,
Unsupervised classification of single particles by cluster tracking in multi-dimensional space, J. Struct. Biol, vol.157, pp.226-239, 2006. ,
Alignment algorithms and per-particle CTF correction for single particle cryo-electron tomography, J. Struct. Biol, vol.194, pp.383-394, 2016. ,
Dynamics of EF-G interaction with the ribosome explored by classification of a heterogeneous cryo-EM dataset, J. Struct. Biol, vol.147, pp.283-290, 2004. ,
Protein complexes in focus, Elife, vol.5, 2016. ,
Measuring the optimal exposure for single particle cryo-EM using a 2.6Å reconstruction of rotavirus VP6, vol.4, p.6980, 2015. ,
The complete structure of the 55S mammalian mitochondrial ribosome, Science, vol.348, pp.303-308, 2015. ,
, Cell, vol.163, pp.1692-1701, 2015.
Implementation of a cryo-electron tomography tilt-scheme optimized for high resolution subtomogram averaging, J. Struct. Biol, vol.16, pp.30113-30117, 2016. ,
Clustering and variance maps for cryo-electron tomography using wedge-masked differences, J. Struct. Biol, vol.175, pp.288-299, 2011. ,
Cellular structural biology as revealed by cryo-electron tomography, J. Cell Sci, vol.129, pp.469-476, 2016. ,
Fast and precise targeting of single tumor cells in vivo by multimodal correlative microscopy, J. Cell Sci, vol.129, pp.444-456, 2016. ,
Purification, characterization and crystallization of the human 80S ribosome, Nucleic Acids Res, vol.42, pp.1-11, 2014. ,
Structure of the human 80S ribosome, Nature, vol.520, pp.640-645, 2015. ,
Subtomogram analysis using the Volta phase plate, J. Struct. Biol, vol.16, pp.30103-30104, 2016. ,
Volta phase plate cryo-EM of the small protein complex Prx3, Nat. Commun, vol.7, p.10534, 2016. ,
Correlative stochastic optical reconstruction microscopy and electron microscopy, PLoS One, vol.10, 2015. ,
Focused ion beam scanning electron microscopy in biology, J. Microsc, vol.254, pp.109-114, 2014. ,
Structure sorting of multiple macromolecular states in heterogeneous cryo-EM samples by 3D multivariate statistical analysis, Open J. Stat, vol.5, pp.820-836, 2015. ,
Visualization of release factor 3 on the ribosome during termination of protein synthesis, Nature, vol.427, pp.862-865, 2004. ,
Correlative cryo-fluorescence light microscopy and cryo-electron tomography of Streptomyces, Methods Cell Biol, vol.124, pp.217-239, 2014. ,
Molecular architecture of the inner ring scaffold of the human nuclear pore complex, Science, vol.352, pp.363-365, 2016. ,
FEI's direct electron detector developments: Embarking on a revolution in cryo-TEM, J. Struct. Biol, vol.192, pp.179-187, 2015. ,
Time-resolved low-dose microscopy of glutamine synthetase molecules, Ultramicroscopy, vol.13, pp.241-252, 1984. ,
Collaborative framework for 3D alignment and classification of heterogeneous subvolumes in cryo-electron tomography, J. Struct. Biol, vol.181, pp.116-127, 2013. ,
Structural analysis of herpes simplex virus by optical super-resolution imaging, Nat. Commun, vol.6, p.5980, 2015. ,
Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM, Nat. Methods, vol.10, pp.584-590, 2013. ,
Efficient estimation of three-dimensional covariance and its application in the analysis of heterogeneous samples in cryo-electron microscopy, Structure, vol.23, pp.1129-1237, 2015. ,
, , vol.352, p.6283, 2016.
Correlative super-resolution fluorescence and electron microscopy of the nuclear pore complex with molecular resolution, J. Cell Sci, vol.127, pp.4351-4355, 2014. ,
Cryo-electron tomography: the challenge of doing structural biology in situ, J. Cell Biol, vol.202, pp.407-419, 2013. ,
Likelihood-based classification of cryo-EM images using FREALIGN, J. Struct. Biol, vol.183, pp.377-388, 2013. ,
Visualizing the molecular sociology at the HeLa cell nuclear periphery, Science, vol.351, pp.969-972, 2016. ,
A focused ion beam milling and lift-out approach for site-specific preparation of frozen-hydrated lamellas from multicellular organisms, J. Struct. Biol, vol.192, pp.262-269, 2015. ,
The palindromic DNA-bound USP/EcR nuclear receptor adopts an asymmetric organization with allosteric domain positioning, Nat. Commun, vol.5, p.4139, 2014. ,
Detective quantum efficiency of electron area detectors in electron microscopy, Ultramicroscopy, vol.109, pp.1126-1143, 2009. ,
Comparison of optimal performance at 300keV of three direct electron detectors for use in low dose electron microscopy, Ultramicroscopy, vol.147, pp.156-163, 2014. ,
Macromolecular architecture in eukaryotic cells visualized by cryoelectron tomography, Science, vol.298, pp.1209-1213, 2002. ,
Integrative structure-function analysis of large nucleoprotein complexes, 2013. ,
Breaking Cryo-EM Resolution Barriers to Facilitate Drug Discovery, Cell, vol.165, pp.1698-1707, 2016. ,
Cryo-electron microscopy -a primer for the non-microscopist, FEBS J, vol.280, pp.28-45, 2013. ,
Zernike phase contrast cryo-electron microscopy and tomography for structure determination at nanometer and subnanometer resolutions, Structure, vol.18, pp.903-912, 2010. ,
The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes, Nat. Commun, vol.5, p.5294, 2014. ,
Single particle and molecular assembly analysis of polyribosomes by single-and double-tilt cryo electron tomography, Ultramicroscopy, vol.126, pp.33-39, 2013. ,
Structure-function insights reveal the human ribosome as a cancer target for antibiotics, Nat. Commun, vol.7, p.12856, 2016. ,
Structure of a bacterial type III secretion system in contact with a host membrane in situ, Nat. Commun, vol.6, p.10114, 2015. ,
Imaging protein three-dimensional nanocrystals with cryo-EM, Acta Crystallogr. D Biol. Crystallogr, vol.69, pp.852-859, 2013. ,
A simple and fast approach for missing-wedge invariant classification of subtomograms extracted from filamentous structures, J. Struct. Biol, vol.16, p.30172, 2016. ,
Structure of the full human RXR/VDR nuclear receptor heterodimer complex with its DR3 target DNA, EMBO J, vol.31, pp.291-300, 2012. ,
Live cell immunogold labelling of RNA polymerase II, Sci. Rep, vol.5, p.8324, 2015. ,
Methods for three-dimensional reconstruction of heterogeneous assemblies, Methods Enzymol, vol.482, pp.321-362, 2010. ,
Mapping 70S ribosomes in intact cells by cryoelectron tomography and pattern recognition, J. Struct. Biol, vol.156, pp.334-341, 2006. ,
A method of focused classification, based on the bootstrap 3D variance analysis, and its application to EF-G-dependent translocation, J. Struct. Biol, vol.154, pp.184-194, 2006. ,
Radiation damage in room-temperature data acquisition with the PILATUS 6M pixel detector, J. Synchrotron Radiat, vol.18, pp.318-328, 2011. ,
Determination of Escherichia coli RNA polymerase structure by single particle cryoelectron microscopy, Methods Enzymol, vol.370, pp.24-42, 2003. ,
A new method for vitrifying samples for cryoEM, J. Struct. Biol, vol.195, pp.190-198, 2016. ,
Towards an optimum design for thin film phase plates, Ultramicroscopy, vol.160, pp.1-6, 2016. ,
Cryo-focused-ion-beam applications in structural biology, Arch. Biochem. Biophys, vol.581, pp.122-130, 2015. ,
Quantitative characterization of electron detectors for transmission electron microscopy, J. Struct. Biol, vol.184, pp.385-393, 2013. ,
Ab initio structure determination from prion nanocrystals at atomic resolution by MicroED, Proc. Natl. Acad. Sci. U.S.A, p.201606287, 2016. ,
Optimized cryo-focused ion beam sample preparation aimed at in situ structural studies of membrane proteins, J. Struct. Biol, vol.16, pp.30151-30154, 2016. ,
Maximum likelihood multi-reference refinement for electron microscopy images, J. Mol. Biol, vol.22, pp.139-149, 2005. ,
Classification of structural heterogeneity by maximum-likelihood methods, Methods Enzymol, vol.482, pp.295-320, 2010. ,
Beam-induced motion correction for sub-megadalton cryo-EM particles, Elife, vol.3, p.3665, 2014. ,
Correlative fluorescence and electron microscopy, Curr. Protoc. Cytom, vol.70, pp.1-10, 2014. ,
New hardware and workflows for semi-automated correlative cryo-fluoresence and cryo-electron microscopy/tomography, J. Struct. Biol, vol.16, pp.30135-30136, 2016. ,
An atomic model of HIV-1 capsid-SP1 reveals structures regulating assembly and maturation, Science, vol.353, pp.506-508, 2016. ,
The collection of MicroED data for macromolecular crystallography, Nat. Protoc, vol.11, pp.895-904, 2016. ,
A maximum-likelihood approach to single-particle image refinement, J. Struct. Biol, vol.122, pp.328-339, 1998. ,
Structure of the protein core of translation initiation factor 2 in apo, GTP-bound and GDP-bound forms, Acta Cryst, vol.69, pp.925-933, 2013. ,
Involvement of IF2 N domain in ribosomal subunit joining revealed from architecture and function of the full-length initiation factor, Proc. Nat. Acad. Sci. U.S.A, vol.110, pp.15656-15661, 2013. ,
Ribosomal 18S rRNA base pairs with mRNA during eukaryotic translation initiation, Nat. Commun, vol.7, p.12622, 2016. ,
URL : https://hal.archives-ouvertes.fr/hal-02294260
Structure of the 30S translation initiation complex, Nature, vol.455, pp.416-420, 2008. ,
URL : https://hal.archives-ouvertes.fr/hal-00342095
Exploiting structure similarity in refinement: automated NCS and target-structure restraints in BUSTER, Acta Crystallogr. D Biol. Crystallogr, vol.68, pp.368-380, 2012. ,
Programming new geometry restraints: parallelity of atomic groups, J. Appl. Crystallogr, vol.48, pp.1130-1141, 2015. ,
The influence of frame alignment with dose compensation on the quality of single particle reconstructions, J. Struct. Biol, vol.192, pp.196-203, 2015. ,
CryoEM structure of the lactococcal siphophage 1358 virion, J. Virol, vol.88, pp.8900-8910, 2014. ,
Maximum likelihood based classification of electron tomographic data, J. Struct. Biol, vol.173, pp.77-85, 2011. ,
Nuclear pore scaffold structure analyzed by super-resolution microscopy and particle averaging, Science, vol.341, pp.655-658, 2013. ,
Automated data collection in single particle electron microscopy, Microscopy (Oxf.), vol.65, pp.43-56, 2016. ,
Single-particle electron cryo-microscopy: towards atomic resolution, Q. Rev. Biophys, vol.33, pp.307-369, 2000. ,
Maximizing the potential of electron cryomicroscopy data collected using direct detectors, J. Struct. Biol, vol.184, pp.193-202, 2013. ,
Opening windows into the cell: focused-ion-beam milling for cryo-electron tomography, Curr. Opin. Struct. Biol, vol.23, pp.771-777, 2013. ,
Towards an optimum design for electrostatic phase plates, Ultramicroscopy, vol.153, pp.22-31, 2015. ,
Cryo-electron tomography and subtomogram averaging, Methods Enzymol, vol.579, pp.329-367, 2016. ,
In vivo virus structures: simultaneous classification, resolution enhancement, and noise reduction in whole-cell electron tomography, J. Struct. Biol, vol.174, pp.425-433, 2011. ,
Recognition and separation of single particles with size variation by statistical analysis of their images, J. Mol. Biol, vol.13, pp.453-460, 2004. ,
, Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine. Elife 3, 2014.
Automated target segmentation and real space fast alignment methods for high-throughput classification and averaging of crowded cryo-electron subtomograms, Bioinformatics, vol.29, pp.274-282, 2013. ,
UCSF Chimera, MODELLER, and IMP: an integrated modeling system, J. Struct. Biol, vol.179, pp.269-278, 2012. ,
An improved cryo-FIB method for fabrication of frozen hydrated lamella, J. Struct. Biol, vol.194, pp.218-223, 2016. ,
Cryo-EM structure of the mature dengue virus at 3.5-Å resolution, Nat. Struct. Mol. Biol, vol.20, pp.105-110, 2013. ,