Skip to Main content Skip to Navigation
Journal articles

Structural features of the salivary gland hypertrophy virus of the tsetse fly revealed by cryo-electron microscopy and tomography

Abstract : Glossina palipides salivary gland hypertrophy virus (GpSGHV) infects tsetse flies, which are vectors for African trypanosomosis. This virus represents a major challenge in insect mass rearing and has hampered the implementation of the sterile insect technique programs in the Member States of the International Atomic Energy Agency. GpSGHV virions consist of long rod-shaped particles over 9000Å in length, but little is known about their detailed structural organization. We show by cryo electron microscopy and cryo electron tomography that the GpSGHV virion has a unique, non-icosahedral helical structure. Its envelope exhibits regularly spaced spikes that protrude from the lipid bilayer and are arranged on a four-start helix. This study provides a detailed insight into the 3D architecture of GpSGHV, which will help to understand the viral life cycle and possibly allow the design of antiviral strategies in the context of tsetse fly infections.
Complete list of metadatas

Cited literature [17 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02409694
Contributor : Bruno Klaholz <>
Submitted on : Friday, December 13, 2019 - 3:12:14 PM
Last modification on : Tuesday, March 17, 2020 - 2:09:19 AM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Collections

Citation

Igor Orlov, Robert Drillien, Daniele Spehner, Max Bergoin, Adly M.M. Abd-Alla, et al.. Structural features of the salivary gland hypertrophy virus of the tsetse fly revealed by cryo-electron microscopy and tomography. Virology, Elsevier, 2018, 514, pp.165-169. ⟨10.1016/j.virol.2017.11.016⟩. ⟨inserm-02409694⟩

Share

Metrics

Record views

163