They were introduced in GSCs using Neon electroporation system according to manufacturer's instructions, pRluc-LC3wt and pRluc-LC3BG120A were a gift from Marja Jaattela, 2008. ,
Z-VRPR-FMK was purchased from Enzo Life Sciences. Q-VD-OPh and tumor necrosis factor-alpha (TNFa) were obtained from R&D Systems. Ionomycin was purchased from Calbiochem. The following primary antibodies were used: NESTIN (Millipore MAB5326), SOX2 (Millipore AB5603), GAPDH (Santa Cruz SC-25778 and SC-32233), TUBULIN (Santa Cruz SC-8035), MALT1 (Santa Cruz SC-46677), EEA1 (BD Bioscience 610456), CTSD (BD Bioscience 610800), PEX1 (BD Bioscience 611719), PECAM (BD Bioscience 557355), TFEB (Bethyl A303-673A), PDI (Abcam ab2792), GM130 (Abcam ab52649), QKI (Atlas HPA019123), CTSD (Atlas HPA063001), ULK1 (Sigma A7481), and FLAG (Sigma F1804). HRP-conjugated secondary antibodies, vol.2, pp.4-5 ,
Lysosomal cell death at a glance, J Cell Sci, vol.126, pp.1905-1912, 2013. ,
Lysosomal mTORC2/PHLPP1/Akt regulate chaperone-mediated autophagy, Mol Cell, vol.59, pp.270-284, 2015. ,
Glioma stem cells promote radioresistance by preferential activation of the DNA damage response, Nature, vol.444, pp.756-760, 2006. ,
High-level nuclear NF-kappa B and Oct-2 is a common feature of cultured Hodgkin/Reed-Sternberg cells, Blood, vol.87, pp.4340-4347, 1996. ,
GlioVis data portal for visualization and analysis of brain tumor expression datasets, Neuro Oncol, vol.19, pp.139-141, 2007. ,
Regression of glioblastoma after chimeric antigen receptor T-cell therapy, N Engl J Med, vol.375, pp.2561-2569, 2016. ,
A perivascular niche for brain tumor stem cells, Cancer Cell, vol.11, pp.69-82, 2007. ,
A restricted cell population propagates glioblastoma growth after chemotherapy, Nature, vol.488, pp.522-526, 2012. ,
Bevacizumab plus radiotherapy-temozolomide for newly diagnosed glioblastoma, N Engl J Med, vol.370, pp.709-722, 2014. ,
Constitutive nuclear factor kappaB activity is required for survival of activated B cell-like diffuse large B cell lymphoma cells, J Exp Med, vol.194, pp.1861-1874, 2001. ,
Targeting the CBM complex causes Treg cells to prime tumours for immune checkpoint therapy, Nature, vol.570, pp.112-116, 2019. ,
The paracaspase MALT1 cleaves the LUBAC subunit HOIL1 during antigen receptor signaling, J Cell Sci, vol.129, pp.1775-1780, 2016. ,
URL : https://hal.archives-ouvertes.fr/inserm-01311283
A catalytic-independent role for the LUBAC in NF-kappaB activation upon antigen receptor engagement and in lymphoma cells, Blood, vol.123, pp.2199-2203, 2014. ,
, The Authors The EMBO Journal, vol.102030, 2019.
The IAP family member BRUCE regulates autophagosome-lysosome fusion, Nat Commun, vol.9, p.599, 2018. ,
Activity-based probes for detection of active MALT1 paracaspase in immune cells and lymphomas, Chem Biol, vol.22, pp.129-138, 2015. ,
Autophagic dysfunction in a lysosomal storage disorder due to impaired proteolysis, Autophagy, vol.9, pp.234-235, 2013. ,
Chapter one -renilla luciferase-LC3B based reporter assay for measuring autophagic flux, Methods Enzymol, vol.588, pp.1-13, 2017. ,
Lysosomal biology in cancer, Methods Mol Biol, vol.1594, pp.293-308, 2017. ,
Secreted factors from brain endothelial cells maintain glioblastoma stem-like cell expansion through the mTOR pathway, EMBO Rep, vol.12, pp.470-476, 2011. ,
4EBP1, a repressor of mRNA translation, is phosphorylated and inactivated by the AKT (PKB) signaling pathway, Genes Dev, vol.12, pp.502-513, 1998. ,
T cell receptor-dependent activation of mTOR signaling in T cells is mediated by Carma1 and MALT1, but not Bcl10, Sci Signal, vol.7, p.55, 2014. ,
Essential role of MALT1 protease activity in activated B cell-like diffuse large B-cell lymphoma, Proc Natl Acad Sci, vol.106, pp.19946-19951, 2009. ,
Pharmacological targeting of apelin impairs glioblastoma growth, Brain, vol.140, pp.2939-2954, 2017. ,
URL : https://hal.archives-ouvertes.fr/inserm-01611131
ELDA: extreme limiting dilution analysis for comparing depleted and enriched populations in stem cell and other assays, J Immunol Methods, vol.347, pp.70-78, 2009. ,
Neutralizing gp130 interferes with endothelial-mediated effects on glioblastoma stem-like cells, Cell Death Differ, vol.24, p.384, 2017. ,
URL : https://hal.archives-ouvertes.fr/inserm-01444543
Cleavage of roquin and regnase-1 by the paracaspase MALT1 releases their cooperatively repressed targets to promote TH17 differentiation, Nat Immunol, vol.15, pp.1079-1089, 2014. ,
Lysosome positioning influences mTORC2 and AKT signaling, Mol Cell, vol.75, pp.26-38, 2019. ,
Targeting glioma stem cells through combined BMI1 and EZH2 inhibition, Nat Med, vol.23, pp.1352-1361, 2017. ,
Holding all the CARDs: how MALT1 controls CARMA/CARD-dependent signaling, Front Immunol, vol.9, p.1927, 2018. ,
NF-kappaB: linking inflammation and immunity to cancer development and progression, Nat Rev Immunol, vol.5, pp.749-759, 2005. ,
MALT1 controls attenuated rabies virus by inducing early inflammation and T cell activation in the brain, J Virol, vol.92, pp.2029-2046, 2018. ,
Lysosomal positioning coordinates cellular nutrient responses, Nat Cell Biol, vol.13, pp.453-460, 2011. ,
Acridine and phenothiazine derivatives as pharmacotherapeutics for prion disease, Proc Natl Acad Sci, vol.98, pp.9836-9841, 2001. ,
Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs, RNA, vol.14, pp.2580-2596, 2008. ,
Cancer stem cells in glioblastoma, Genes Dev, vol.29, pp.1203-1217, 2015. ,
Vulnerability of invasive glioblastoma cells to lysosomal membrane destabilization, EMBO Mol Med, vol.11, p.9034, 2019. ,
Association of constitutive nuclear factor-kappaB activation with aggressive aspects and poor prognosis in cervical cancer, Int J Gynecol Cancer, vol.19, pp.1421-1426, 2009. ,
PI3K/Akt/ mTOR signaling pathway and targeted therapy for glioblastoma, Oncotarget, vol.7, pp.33440-33450, 2016. ,
Treatment of schizophrenia, Br Med J, vol.2, pp.78-80, 1957. ,
Defining and measuring autophagosome flux-concept and reality, Autophagy, vol.10, pp.2087-2096, 2014. ,
Principles of cancer therapy: oncogene and non-oncogene addiction, Cell, vol.138, p.807, 2009. ,
Salinomycin kills cancer stem cells by sequestering iron in lysosomes, Nat Chem, vol.10, pp.1025-1033, 2017. ,
URL : https://hal.archives-ouvertes.fr/hal-01787681
Hypoxic induction of vasorin regulates Notch1 turnover to maintain glioma stem-like cells, Cell Stem Cell, vol.22, pp.104-118, 2017. ,
MALT1 is a critical mediator of PAR1-driven NF-jB activation and metastasis in multiple tumor types, 2019. ,
, Oncogene
Pharmacological inhibition of MALT1 protease activity protects mice in a mouse model of multiple sclerosis, J Neuroinflammation, vol.11, p.124, 2014. ,
Mepazine inhibits RANK-induced osteoclastogenesis independent of its MALT1 inhibitory function, Beyaert R, vol.23, p.3144, 2018. ,
Pharmacologic inhibition of MALT1 protease by phenothiazines as a therapeutic approach for the treatment of aggressive ABC-DLBCL, Cancer Cell, vol.22, pp.825-837, 2012. ,
Inflammatory T cell responses rely on amino acid transporter ASCT2 facilitation of glutamine uptake and mTORC1 kinase activation, Immunity, vol.40, pp.692-705, 2014. ,
A loss-of-function RNA interference screen for molecular targets in cancer, Nature, vol.441, pp.106-110, 2006. ,
Repositioning chlorpromazine for treating chemoresistant glioma through the inhibition of cytochrome c oxidase bearing the COX4-1 regulatory subunit, Oncotarget, vol.8, pp.37568-37583, 2017. ,
Osteopontin-CD44 signaling in the glioma perivascular niche enhances cancer stem cell phenotypes and promotes aggressive tumor growth, Cell Stem Cell, vol.14, pp.357-369, 2014. ,
4E-BP1, a multifactor regulated multifunctional protein, Cell Cycle, vol.15, pp.781-786, 2016. ,
Regulation of hypoxiainduced autophagy in glioblastoma involves ATG9A, Br J Cancer, vol.117, pp.813-825, 2017. ,
The proteolytic activity of the paracaspase MALT1 is key in T cell activation, Nat Immunol, vol.9, pp.272-281, 2008. ,
Bcl10-controlled Malt1 paracaspase activity is key for the immune suppressive function of regulatory T cells, Nat Commun, vol.10, p.2352, 2019. ,
A gene network regulating lysosomal biogenesis and function, Science, vol.325, pp.473-477, 2009. ,
Structural analysis of phenothiazine derivatives as allosteric inhibitors of the MALT1 paracaspase, Angew Chem Int Ed Engl, vol.52, pp.10384-10387, 2013. ,
A lysosome-to-nucleus signalling mechanism senses and regulates the lysosome via mTOR and TFEB, EMBO J, vol.31, pp.1095-1108, 2012. ,
Dual targeting of autophagic regulatory circuitry in gliomas with repurposed drugs elicits cell-lethal autophagy and therapeutic benefit, Cancer Cell, vol.28, pp.456-471, 2015. ,
Qki deficiency maintains stemness of glioma stem cells in suboptimal environment by downregulating endolysosomal degradation, Nat Genet, vol.49, pp.75-86, 2016. ,
Identification of human brain tumour initiating cells, Nature, vol.432, pp.396-401, 2004. ,
T-cell receptor-induced JNK activation requires proteolytic inactivation of CYLD by MALT1: CYLD is cleaved by MALT1, 2011. ,
, EMBO J, vol.30, pp.1742-1752
Oncogenic activation of NF-kappaB, Cold Spring Harb Perspect Biol, vol.2, p.109, 2010. ,
Effects of radiotherapy with concomitant and adjuvant temozolomide versus radiotherapy alone on survival in glioblastoma in a randomised phase III study: 5-year analysis of the EORTC-NCIC trial, Lancet Oncol, vol.10, pp.459-466, 2009. ,
Maintenance therapy with tumor-treating fields plus temozolomide vs temozolomide alone for glioblastoma: a randomized clinical trial, JAMA, vol.314, pp.2535-2543, 2015. ,
Drug repositioning in glioblastoma: a pathway perspective, Front Pharmacol, vol.9, p.218, 2018. ,
Post-translational modifications of the CARMA1-BCL10-MALT1 complex in lymphocytes and activated B-cell like subtype of diffuse large B-cell lymphoma, Front Oncol, vol.8, p.498, 2018. ,
URL : https://hal.archives-ouvertes.fr/inserm-01908802
Extracellular vesicletransported Semaphorin3A promotes vascular permeability in glioblastoma, Oncogene, vol.35, pp.2615-2623, 2016. ,
URL : https://hal.archives-ouvertes.fr/inserm-01406334
Distinct neural stem cells proliferate in response to EGF and FGF in the developing mouse telencephalon, Dev Biol, vol.208, pp.166-188, 1999. ,
Malt1-induced cleavage of Regnase-1 in CD4 + helper T cells regulates immune activation, Cell, vol.153, pp.1036-1049, 2013. ,
Lysosome-dependent cell death and deregulated autophagy induced by amine-modified polystyrene nanoparticles, Open Biol, vol.8, p.170271, 2018. ,
Reciprocal signaling between glioblastoma stem cells and differentiated tumor cells promotes malignant progression, Cell Stem Cell, vol.22, pp.514-528, 2018. ,
Re-purposing chloroquine for glioblastoma: potential merits and confounding variables, Front Oncol, vol.8, p.335, 2018. ,
The quaking 1-5 protein (QKI-5) has a novel nuclear localization signal and shuttles between the nucleus and the cytoplasm, J Biol Chem, vol.274, pp.29202-29210, 1999. ,
The evolving landscape of glioblastoma stem cells, Curr Opin Neurol, vol.26, pp.701-707, 2013. ,
Termination of autophagy and reformation of lysosomes regulated by mTOR, Nature, vol.465, pp.942-946, 2010. ,
Loperamide, pimozide, and STF-62247 trigger autophagy-dependent cell death in glioblastoma cells, The Authors The EMBO Journal, vol.9, 2018. ,