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nanoPaint: a Tool for Rapid and Dynamic Imaging of Membrane Structural Plasticity at the Nano- Scale

Abstract : Single-particle tracking with quantum dots (QDs) constitutes a powerful tool to track the nanoscopic dynamics of individual cell membrane components unveiling their membrane diffusion characteristics. Here, the nano-resolved population dynamics of QDs is exploited to reconstruct the topography and structural changes of the cell membrane surface with high temporal and spatial resolution. For this proof-of-concept study, bright, small and stable biofunctional QD nanoconstructs are utilized recognizing the endogenous neuronal cannabinoid type-1 receptor, a highly-expressed and fast-diffusing membrane protein, together with a commercial point-localization microscope. Rapid QD diffusion on the axonal plasma membrane of cultured 2 hippocampal neurons allows precise reconstruction of the membrane surface in less than one minute with a spatial resolution of tens of nanometers. Access of the QD nanoconstructs to the synaptic cleft enables rapid 3D topological reconstruction of the entire presynaptic component. Successful reconstruction of membrane nano-topology and deformation at the second timescale is also demonstrated for HEK293 cell filopodia and axons. Named "nanoPaint", this super-resolution imaging technique amenable to any endogenous transmembrane target represents then a versatile platform to rapidly and accurately reconstruct the cell membrane nano-topography, thereby enabling the study of the rapid dynamic phenomena involved in neuronal membrane plasticity.
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Submitted on : Monday, December 2, 2019 - 7:03:10 PM
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Mariana Tasso, Thomas Pons, Nicolas Lequeux, Diana Zala, Zsolt D Lenkei, et al.. nanoPaint: a Tool for Rapid and Dynamic Imaging of Membrane Structural Plasticity at the Nano- Scale. Small, Wiley-VCH Verlag, 2019, 15 (47), pp.e1902796. ⟨10.1002/smll.201902796⟩. ⟨inserm-02390138⟩



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