Holographic Traction Force Microscopy

Abstract : Traction Force Microscopy (TFM) computes the forces exerted at the surface of an elastic material by measuring induced deformations in volume. It is used to determine the pattern of the adhesion forces exerted by cells or by cellular assemblies grown onto a soft deformable substrate. Typically, colloidal particles are dispersed in the substrate and their displacement is monitored by fluorescent microscopy. As with any other fluorescent techniques, the accuracy in measuring a particule's position is ultimately limited by the number of evaluated fluorescent photons. Here, we present a TFM technique based on the detection of probe particle displacements by holographic tracking microscopy. We show that nanometer scale resolutions of the particle displacements can be obtained and determine the maximum volume fraction of markers in the substrate. We demonstrate the feasibility of the technique experimentally and measure the three-dimensional force fields exerted by colorectal cancer cells cultivated onto a polyacrylamide gel substrate.
Complete list of metadatas

Cited literature [54 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02364168
Contributor : Christian Gaiddon <>
Submitted on : Thursday, November 14, 2019 - 5:21:03 PM
Last modification on : Saturday, November 16, 2019 - 1:44:06 AM

File

s41598-018-21206-2.pdf
Publisher files allowed on an open archive

Identifiers

Citation

Stanislaw Makarchuk, Nicolas Beyer, Christian Gaiddon, Wilfried Grange, Pascal Hebraud. Holographic Traction Force Microscopy. Scientific Reports, Nature Publishing Group, 2018, 8 (1), pp.3038. ⟨10.1038/s41598-018-21206-2⟩. ⟨inserm-02364168⟩

Share

Metrics

Record views

41

Files downloads

36