, Cells were grown in RPMI (Roswell Park Memorial Institute medium, Dominique DUTSCHER SAS-Head Office: 30, rue de l'Industrie-BP62-67172 BRUMATH cedex, France) with 10% fetal bovine serum, Materials AGS and KATOIII cells were obtained from ATCC (ATCC ® : LGC Standards S.a.r.l. 6, rue Alfred Kastler BP 83076 F-67123 Molsheim Cedex France: CRL-1739?, HTB-103 TM )
64293 Darmstadt, Germany) and 1% penicillin/streptomycin (PAN-Biotech, Mycoplasma contamination was tested negative using PlasmoTest, vol.13, p.31400, 200152. ,
Healthy tissue samples, gastric tumor biopsies, and distant normal gastric tissues (n = 26) were obtained from the Digestive Surgery department of Hautepierre Hospital (CHU Hautepierre, Strasbourg 67200, France, authorization number: NCT02491840) or the National Cancer Research Center, EST4: IDRCB2015-A00198-41/PRI2014-HUSn ? 6042) by the Human Ethics Committee of the Strasbourg University Hospital ,
, Cell survival Assay A total of 10,000 cells were seeded per well in 96-well microplates (Falcon Multiwell, Dutscher: 30, rue de l'Industrie-BP62, 67170 Brumath, France), 24 h prior to any treatment. Cisplatin was applied for 48 h in fresh medium. MTT assay was performed as previously described by replacing the medium with fresh medium supplemented with 5 mg/L MTT (Sigma, Lyon-Saint Exupéry BP 113, 69125 Lyon, France) for 1 h [50]. Cells were lysed in in DMSO 100% (100 µL/wells). Measurements were performed at 550 nm with the Tristar 2 Multimode Reader
Recent developments and innovations in gastric cancer, World J. Gastroenterol, vol.22, pp.4307-4320, 2016. ,
Cisplatin as an anti-tumor drug: Cellular mechanisms of activity, drug resistance and induced side effects, Cancers, vol.3, pp.1351-1371, 2011. ,
Cisplatin resistance in gastric cancer cells is associated with HER2 upregulation-induced epithelial-mesenchymal transition, Sci. Rep, 2016. ,
A review on biomarkers for prediction of treatment outcome in gastric cancer, Anticancer Res, vol.33, pp.1257-1266, 2013. ,
Gastric cancer pathology and underlying molecular mechanisms, Dig. Surg, vol.30, pp.150-158, 2013. ,
Molecular events in gastric carcinogenesis, J. Med. Life, vol.7, pp.375-378, 2014. ,
Mutations in the p53 tumor suppressor gene: Important milestones at the various steps of tumorigenesis, Genes Cancer, vol.2, pp.466-474, 2011. ,
Evolutionary action score of TP53 coding variants is predictive of platinum response in head and neck cancer patients, Cancer Res, vol.75, pp.1205-1215, 2015. ,
Mutant p53 gain-of-function in cancer, Cold Spring Harb. Perspect. Biol, 1107. ,
A subset of tumor-derived mutant forms of p53 down-regulate p63 and p73 through a direct interaction with the p53 core domain, Mol. Cell Biol, vol.21, pp.1874-1887, 2001. ,
URL : https://hal.archives-ouvertes.fr/inserm-02360323
Structure and function in the p53 family, Cell Death Differ, vol.6, pp.1169-1173, 1999. ,
The role of p53 protein family in gastrointestinal malignancies, Cell Death Differ, vol.13, pp.935-940, 2006. ,
Zaika, A.I. p73 isoforms can induce T-cell factor-dependent transcription in gastrointestinal cells, Cancer Res, vol.64, pp.6390-6393, 2004. ,
The gastrin gene promoter is regulated by p73 isoforms in tumor cells, Oncogene, vol.25, pp.6032-6036, 2006. ,
Characterization of DeltaNp73 expression and regulation in gastric and esophageal tumors, Oncogene, vol.29, pp.5861-5868, 2010. ,
Epigenetics in cancer: Targeting chromatin modifications, Mol. Cancer Ther, vol.8, pp.1409-1420, 2009. ,
Function of miRNA in controlling drug resistance of human cancers, Curr. Drug Targets, vol.14, pp.1118-1127, 2013. ,
The biology of HDAC in cancer: The nuclear and epigenetic components, Handb. Exp. Pharmacol, vol.206, pp.13-37, 2011. ,
Histone deacetylases (HDACs): Characterization of the classical HDAC family, Biochem. J, vol.370, pp.737-749, 2003. ,
Histone deacetylase (HDAC) 1 and 2 expression and chemotherapy in gastric cancer, Ann. Surg. Oncol, vol.17, pp.3336-3343, 2010. ,
Histone deacetylase HDAC4 promotes gastric cancer SGC-7901 cells progression via p21 repression, PLoS ONE, vol.9, 2014. ,
Histone deacetylases specifically down-regulate p53-dependent gene activation, J. Biol. Chem, vol.275, pp.20436-20443, 2000. ,
Emerging role of miRNAs in the drug resistance of gastric cancer, Int. J. Mol. Sci, vol.17, 2016. ,
Significance of microRNA 21 in gastric cancer, Clin. Res. Hepatol. Gastroenterol, 2016. ,
Role of microRNAs in gastric cancer, World J. Gastroenterol, vol.20, pp.5694-5699, 2014. ,
Functional links between clustered microRNAs: Suppression of cell-cycle inhibitors by microRNA clusters in gastric cancer, Nucl. Acids Res, vol.37, pp.1672-1681, 2009. ,
Decreased expression of microRNA-143 and -145 in human gastric cancers, Oncology, vol.77, pp.12-21, 2009. ,
Clinical significance of miR-144-ZFX axis in disseminated tumour cells in bone marrow in gastric cancer cases, Br. J. Cancer, vol.107, pp.1345-1353, 2012. ,
Fan, D. miR-15b and miR-16 modulate multidrug resistance by targeting BCL2 in human gastric cancer cells, Int. J. Cancer, vol.123, pp.372-379, 2008. ,
A ruthenium anticancer compound interacts with histones and impacts differently on epigenetic and death pathways compared to cisplatin, Oncotarget, 2016. ,
Epigenetic inactivation of the putative DNA/RNA helicase SLFN11 in human cancer confers resistance to platinum drugs, Oncotarget, vol.7, pp.3084-3097, 2016. ,
Regulation of class IIa HDAC activities: It is not only matter of subcellular localization, Epigenomics, vol.8, pp.251-269, 2016. ,
Histone deacetylase (HDAC) inhibitors with a novel connecting unit linker region reveal a selectivity profile for HDAC4 and HDAC5 with improved activity against chemoresistant cancer cells, J. Med. Chem, vol.56, pp.427-436, 2013. ,
Theoretical basis, experimental design, and computerized simulation of synergism and antagonism in drug combination studies, Pharmacol. Rev, vol.58, pp.621-681, 2006. ,
Specific activity of class II histone deacetylases in human breast cancer cells, Mol. Cancer Res, vol.6, pp.1908-1919, 2008. ,
URL : https://hal.archives-ouvertes.fr/inserm-00351047
Molecular classifications of gastric cancers: Novel insights and possible future applications, World J. Gastrointest. Oncol, vol.9, pp.194-208, 2017. ,
Fahraeus, R. p53-mediated suppression of BiP triggers BIK-induced apoptosis during prolonged endoplasmic reticulum stress, Cell Death Differ, vol.24, pp.1717-1729, 2017. ,
Nuclear translocation of annexin 1 following oxygen-glucose deprivation-reperfusion induces apoptosis by regulating Bid expression via p53 binding, Cell Death Dis, vol.7, 2016. ,
Inhibition of histone deacetylase 4 increases cytotoxicity of docetaxel in gastric cancer cells, Proteomics Clin. Appl, vol.8, pp.924-931, 2014. ,
Overexpressed HDAC4 is associated with poor survival and promotes tumor progression in esophageal carcinoma, Aging, vol.8, pp.1236-1249, 2016. ,
Genomic pathway analysis reveals that EZH2 and HDAC4 represent mutually exclusive epigenetic pathways across human cancers, BMC Med. Genom, vol.6, 2013. ,
Histone deacetylase 4 increases progressive epithelial ovarian cancer cells via repression of p21 on fibrillar collagen matrices, Oncol. Rep, vol.35, pp.948-954, 2016. ,
Therapeutic targeting of miR-29b/HDAC4 epigenetic loop in multiple myeloma, Mol. Cancer Ther, 2016. ,
Transcriptional repression by the HDAC4-RelB-p52 complex regulates multiple myeloma survival and growth, Nat. Commun, vol.6, 2015. ,
HDAC4 promotes growth of colon cancer cells via repression of p21, Mol. Biol. Cell, vol.19, pp.4062-4075, 2008. ,
Mechanism of chemoresistance mediated by miR-140 in human osteosarcoma and colon cancer cells, Oncogene, vol.28, pp.4065-4074, 2009. ,
Direct p53 transcriptional repression: In vivo analysis of CCAAT-containing G2/M promoters, Mol. Cell Biol, vol.25, pp.3737-3751, 2005. ,
DNA damage promotes histone deacetylase 4 nuclear localization and repression of G2/M promoters, via p53 C-terminal lysines, J. Biol. Chem, vol.281, pp.2347-2357, 2006. ,
Histone deacetylase 4 interacts with 53BP1 to mediate the DNA damage response, J. Cell Biol, vol.160, pp.1017-1027, 2003. ,
The tissue-specific transcription factor Pit-1/GHF-1 binds to the c-fos serum response element and activates c-fos transcription, Mol. Endocrinol, vol.13, pp.742-751, 1999. ,
Induction of caspase 8 and reactive oxygen species by ruthenium-derived anticancer compounds with improved water solubility and cytotoxicity, Biochem. Pharmacol, vol.84, pp.1428-1436, 2012. ,
Ca2+/calmodulin kinase types II and IV regulate c-fos transcription in the AtT20 corticotroph cell line, Mol. Cell Endocrinol, vol.120, pp.1-8, 1996. ,
Complex regulation of p73 isoforms after alteration of amyloid precursor polypeptide (APP) function and DNA damage in neurons, J. Biol. Chem, vol.286, pp.43013-43025, 2011. ,
AltAnalyze and DomainGraph: Analyzing and visualizing exon expression data, Nucl. Acids Res, vol.38, pp.755-762, 2010. ,