Development and Evaluation of a Duo Chikungunya Virus Real-Time RT-PCR Assay Targeting Two Regions within the Genome

Abstract : Chikungunya virus (CHIKV) re-emerged as a globalized health threat fifteen years ago. There are dozens of RT-PCR assays published. An inventory of the latter was made, and after in silico analysis, two assays were selected for their ability to detect strains belonging to the five CHIKV genetic lineages. They were combined in order to provide a robust assay not affected by genetic point mutations and the resulting Duo CHIKV real-time RT-PCR assay was compared to the two parental single-plex tests against five strains belonging to the five genetic lineages. The Duo CHIKV assay performed equally, or better, in terms of sensitivity, specificity, linearity and signal intensity. Dual-target assays are better suited for viruses having the propensity to evolve into new variants via point mutations or major sequence deletions/insertions. Here, we demonstrated that combining two single systems into a dual-target assay did not impair sensitivity and specificity, and proved a potent diagnostic tool to face a potential emergence of CHIKV variants by newly evolving mutations.
Complete list of metadatas

Cited literature [29 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-02353622
Contributor : Antoine Nougairede <>
Submitted on : Thursday, November 7, 2019 - 1:35:53 PM
Last modification on : Tuesday, November 12, 2019 - 10:02:36 PM

File

viruses-11-00755.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Laurence Thirion, Laura Pezzi, Iban Corcostegui, Audrey Dubot-Pérès, Alessandra Falchi, et al.. Development and Evaluation of a Duo Chikungunya Virus Real-Time RT-PCR Assay Targeting Two Regions within the Genome. Viruses, MDPI, 2019, 11 (8), pp.755. ⟨10.3390/v11080755⟩. ⟨inserm-02353622⟩

Share

Metrics

Record views

152

Files downloads

6