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Knockdown of human AMPK using the CRISPR-Cas9 genome-editing system

Abstract : AMP activated protein kinase (AMPK) is a critical energy sensor, regulating signaling networks involved in pathology including metabolic diseases and cancer. This increasingly recognized role of AMPK has prompted tremendous research efforts to develop new pharmacological AMPK activators. To precisely study the role of AMPK, and the specificity and activity of AMPK activators in cellular models, genetic AMPK inactivating tools are required. We report here methods for genetic inactivation of AMPK 1/ 2 catalytic subunits in human cell lines by the CRISPR/Cas9 technology, a recent breakthrough technique for genome editing.
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Contributor : Benoit Viollet Connect in order to contact the contributor
Submitted on : Tuesday, November 5, 2019 - 7:56:45 PM
Last modification on : Tuesday, November 16, 2021 - 4:00:06 AM
Long-term archiving on: : Friday, February 7, 2020 - 1:04:42 AM



Adrien Grenier, Pierre Sujobert, Séverine Olivier, Hélène Guermouche, Johanna Mondesir, et al.. Knockdown of human AMPK using the CRISPR-Cas9 genome-editing system. Methods Mol Biol, 1732, pp.171-194, 2018, ⟨10.1007/978-1-4939-7598-3_11⟩. ⟨inserm-02349913⟩



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