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Journal articles
Rapid immunoassay exploiting nanoparticles and micromagnets: proof-of-concept using ovalbumin model
Abstract : AIM:
We present a fast magnetic immunoassay, combining magnetic nanoparticles and micromagnets. High magnetic field gradients from micromagnets are used to develop a new approach to the standard ELISA. Materials & methods/results: A proof-of-concept based on colorimetric quantification of antiovalbumin antibody in buffer is performed and compared with an ELISA. After optimization, the magnetic immunoassay exhibits a limit of detection (40 ng/ml) and a dynamic range (40-2500 ng/ml) similar to that of ELISAs developed using same biochemical tools.
CONCLUSION:
Micromagnets can be fully integrated in multiwell plates at low cost to allow the efficient capture of immunocomplexes carried by magnetic nanoparticles. The method is generic and permits to perform magnetic ELISA in 30 min.
Cited literature [24 references]
https://www.hal.inserm.fr/inserm-02333922
Contributor : Patrice Marche Connect in order to contact the contributor
Submitted on : Friday, October 25, 2019 - 4:23:10 PM
Last modification on : Wednesday, October 27, 2021 - 7:50:19 AM
Long-term archiving on: : Sunday, January 26, 2020 - 5:22:40 PM
Contributor : Patrice Marche Connect in order to contact the contributor
Submitted on : Friday, October 25, 2019 - 4:23:10 PM
Last modification on : Wednesday, October 27, 2021 - 7:50:19 AM
Long-term archiving on: : Sunday, January 26, 2020 - 5:22:40 PM
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