Rapid immunoassay exploiting nanoparticles and micromagnets: proof-of-concept using ovalbumin model

AIM: We present a fast magnetic immunoassay, combining magnetic nanoparticles and micromagnets. High magnetic field gradients from micromagnets are used to develop a new approach to the standard ELISA. Materials & methods/results: A proof-of-concept based on colorimetric quantification of antiovalbumin antibody in buffer is performed and compared with an ELISA. After optimization, the magnetic immunoassay exhibits a limit of detection (40 ng/ml) and a dynamic range (40-2500 ng/ml) similar to that of ELISAs developed using same biochemical tools. CONCLUSION: Micromagnets can be fully integrated in multiwell plates at low cost to allow the efficient capture of immunocomplexes carried by magnetic nanoparticles. The method is generic and permits to perform magnetic ELISA in 30 min.

Keywords

method development magnetism immunoassay ovalbumin nanoparticles colorimetry diagnostic ELISA

Domains

Life Sciences [q-bio] Biotechnology Life Sciences [q-bio] Immunology

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https://www.hal.inserm.fr/inserm-02333922

Submitted on : Friday, October 25, 2019-4:23:10 PM

Last modification on : Friday, January 7, 2022-3:47:39 AM

Long-term archiving on: Sunday, January 26, 2020-5:22:40 PM

Dates and versions

inserm-02333922 , version 1 (25-10-2019)

Identifiers

HAL Id : inserm-02333922 , version 1
DOI : 10.4155/bio-2016-0232
PUBMED : 28225302

Cite

Sarah Delshadi, Guillaume Blaire, Paul Kauffmann, Mario Fratzl, Thibaut Devillers, et al.. Rapid immunoassay exploiting nanoparticles and micromagnets: proof-of-concept using ovalbumin model. Bioanalysis, 2017, 9 (6), pp.517-526. ⟨10.4155/bio-2016-0232⟩. ⟨inserm-02333922⟩

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