Abstract : AIM:
We present a fast magnetic immunoassay, combining magnetic nanoparticles and micromagnets. High magnetic field gradients from micromagnets are used to develop a new approach to the standard ELISA. Materials & methods/results: A proof-of-concept based on colorimetric quantification of antiovalbumin antibody in buffer is performed and compared with an ELISA. After optimization, the magnetic immunoassay exhibits a limit of detection (40 ng/ml) and a dynamic range (40-2500 ng/ml) similar to that of ELISAs developed using same biochemical tools.
CONCLUSION:
Micromagnets can be fully integrated in multiwell plates at low cost to allow the efficient capture of immunocomplexes carried by magnetic nanoparticles. The method is generic and permits to perform magnetic ELISA in 30 min.
https://www.hal.inserm.fr/inserm-02333922 Contributor : Patrice MARCHEConnect in order to contact the contributor Submitted on : Friday, October 25, 2019 - 4:23:10 PM Last modification on : Friday, January 7, 2022 - 3:47:39 AM Long-term archiving on: : Sunday, January 26, 2020 - 5:22:40 PM
File
Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed
until : jamais