Rapid immunoassay exploiting nanoparticles and micromagnets: proof-of-concept using ovalbumin model

Sarah Delshadi 1, 2 Guillaume Blaire 1 Paul Kauffmann 1 Mario Fratzl 3 Thibaut Devillers 3 Didier Delabouglise 4 Marianne Weidenhaupt 4 Nora Dempsey 3 Orphée Cugat 1 Franz Bruckert 4 Patrice Marche 2, *
* Corresponding author
1 G2ELab - Laboratoire de Génie Electrique de Grenoble
2 IAB - Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble)
3 MNM - Micro et NanoMagnétisme
NEEL - Institut Néel
4 LMGP - Laboratoire des matériaux et du génie physique
Abstract : AIM: We present a fast magnetic immunoassay, combining magnetic nanoparticles and micromagnets. High magnetic field gradients from micromagnets are used to develop a new approach to the standard ELISA. Materials & methods/results: A proof-of-concept based on colorimetric quantification of antiovalbumin antibody in buffer is performed and compared with an ELISA. After optimization, the magnetic immunoassay exhibits a limit of detection (40 ng/ml) and a dynamic range (40-2500 ng/ml) similar to that of ELISAs developed using same biochemical tools. CONCLUSION: Micromagnets can be fully integrated in multiwell plates at low cost to allow the efficient capture of immunocomplexes carried by magnetic nanoparticles. The method is generic and permits to perform magnetic ELISA in 30 min.
Keywords : ovalbumin nanoparticles colorimetry diagnostic ELISA immunoassay magnetism method development
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https://www.hal.inserm.fr/inserm-02333922
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Submitted on : Friday, October 25, 2019 - 4:23:10 PM
Last modification on : Wednesday, November 6, 2019 - 1:38:50 AM

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Sarah Delshadi, Guillaume Blaire, Paul Kauffmann, Mario Fratzl, Thibaut Devillers, et al.. Rapid immunoassay exploiting nanoparticles and micromagnets: proof-of-concept using ovalbumin model. Bioanalysis, 2017, 9 (6), pp.517-526. ⟨10.4155/bio-2016-0232⟩. ⟨inserm-02333922⟩

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