Overcoming immunogenicity issues of HIV p24 antigen by the use of innovative nanostructured lipid carriers as delivery systems: evidences in mice and non-human primates
Résumé
HIV is one of the deadliest pandemics of modern times, having already caused 35 million deaths around the world. Despite the
huge efforts spent to develop treatments, the virus cannot yet be eradicated and continues to infect new people. Spread of the
virus remains uncontrolled, thus exposing the worldwide population to HIV danger, due to the lack of efficient vaccines. The latest
clinical trials describe the challenges associated with developing an effective prophylactic HIV vaccine. These immunological
obstacles will only be overcome by smart and innovative solutions applied to the design of vaccine formulations. Here, we describe
the use of nanostructured lipid carriers (NLC) for the delivery of p24 protein as a model HIV antigen, with the aim of increasing its
immunogenicity. We have designed vaccine formulations comprising NLC grafted with p24 antigen, together with cationic NLC
optimized for the delivery of immunostimulant CpG. This tailored system significantly enhanced immune responses against p24, in
terms of specific antibody production and T-cell activation in mice. More importantly, the capacity of NLC to induce specific
immune responses against this troublesome HIV antigen was further supported by a 7-month study on non-human primates (NHP).
This work paves the way toward the development of a future HIV vaccine, which will also require the use of envelope antigens.
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...