Detection of free-circulating tumor-associated DNA in plasma of colorectal cancer patients and its association with prognosis, Int. J. Cancer, vol.100, pp.542-548, 2002. ,
Amplification of complex gene libraries by emulsion PCR, Nat. Methods, vol.3, pp.545-550, 2006. ,
Digital quantification of mutant DNA in cancer patients, Curr. Opin. Oncol, vol.19, pp.36-42, 2007. ,
Selected technologies for measuring acquired genetic damage in humans, Environ. Mol. Mutagen, vol.51, pp.851-870, 2010. ,
The cancer biomarker problem, Nature, vol.452, pp.548-552, 2008. ,
Microfluidic single cell analysis: from promise to practice, Curr. Opin. Chem. Biol, 2012. ,
Quantitation of targets for PCR by use of limiting dilution, Biotechniques, vol.13, pp.444-449, 1992. ,
Human immunodeficiency virus-infected individuals contain provirus in small numbers of peripheral mononuclear cells and at low copy numbers, J. Virol, vol.64, pp.864-872, 1990. ,
, Digital PCR. Proc. Natl. Acad. Sci. U.S.A, vol.96, pp.9236-9241, 1999.
Detection of proximal colorectal cancers through analysis of faecal DNA, Lancet, vol.359, pp.403-404, 2002. ,
Principle and applications of digital PCR, Expert Rev. Mol. Diagn, vol.4, pp.41-47, 2004. ,
Assessment of plasma DNA levels, allelic imbalance, and CA 125 as diagnostic tests for cancer, J. Natl. Cancer Inst, vol.94, pp.1697-1703, 2002. ,
Detection of allelic imbalance in ascitic supernatant by digital single nucleotide polymorphism analysis, Clin. Cancer Res, vol.8, pp.2580-2585, 2002. ,
Evidence that genetic instability occurs at an early stage of colorectal tumorigenesis, Cancer Res, vol.61, pp.818-822, 2001. ,
Counting alleles reveals a connection between chromosome 18q loss and vascular invasion, Nat. Biotechnol, vol.19, pp.78-81, 2001. ,
Counting alleles to predict recurrence of earlystage colorectal cancers, Lancet, vol.359, pp.219-225, 2002. ,
Diverse tumorigenic pathways in ovarian serous carcinoma, Am. J. Pathol, vol.160, pp.1223-1228, 2002. ,
Mutations in BRAF and KRAS characterize the development of low-grade ovarian serous carcinoma, J. Natl. Cancer Inst, vol.95, pp.484-486, 2003. ,
Top-down morphogenesis of colorectal tumors, Proc. Natl. Acad. Sci. U.S.A, vol.98, pp.2640-2645, 2001. ,
Small changes in expression affect predisposition to tumorigenesis, Nat. Genet, vol.30, pp.25-26, 2002. ,
Colorectal cancer screening: prospects for molecular stool analysis, Nat. Rev. Cancer, vol.5, pp.199-209, 2005. ,
KRAS mutation detection trap, J. Clin. Oncol, vol.29, pp.208-209, 2011. ,
Should oncologists be aware in their clinical practice of K-ras molecular analysis?, J. Clin. Oncol, vol.29, pp.206-207, 2011. ,
Tiny droplets for high-throughput cell-based assays, Unravelling More of Cell Genomics: Let's Use the Micro and Nanotools!, pp.261-284, 2010. ,
URL : https://hal.archives-ouvertes.fr/hal-02149103
A massively parallel PicoTiterPlate based platform for discrete picoliter-scale polymerase chain reactions, Electrophoresis, vol.24, pp.3769-3777, 2003. ,
Nanoliter high throughput quantitative PCR, Nucleic Acids Res, vol.34, p.123, 2006. ,
Cloning of RNA molecules in vitro, Nucleic Acids Res, vol.21, pp.2349-2353, 1993. ,
In situ localized amplification and contact replication of many individual DNA molecules, Nucleic Acids Res, vol.27, p.34, 1999. ,
From micro-to nanofabrication with soft materials, Science, vol.290, pp.1536-1540, 2000. ,
Single-molecule DNA amplification and analysis using microfluidics, Chem. Rev, vol.110, pp.4910-4947, 2010. ,
High throughput gene expression measurement with real time PCR in a microfluidic dynamic array, PLoS ONE, vol.3, p.1662, 2008. ,
Isolation and characterization of endosteal niche cell populations that regulate hematopoietic stem cells, Blood, vol.116, pp.1422-1432, 2010. ,
Studying copy number variations using a nanofluidic platform, Nucleic Acids Res, vol.36, p.116, 2008. ,
Molecular copy-number counting: potential of single-molecule diagnostics, Expert Rev. Mol. Diagn, vol.9, pp.309-312, 2009. ,
Single molecule detection in nanofluidic digital array enables accurate measurement of DNA copy number, Anal. Bioanal. Chem, vol.394, pp.457-467, 2009. ,
Quantitative miRNA expression analysis using fluidigm microfluidics dynamic arrays, BMC Genomics, vol.12, p.144, 2011. ,
Microfluidic-based multiplex qRT-PCR identifies diagnostic and prognostic microRNA signatures in the sera of prostate cancer patients, Cancer Res, vol.71, pp.550-560, 2011. ,
Comprehensive microRNA expression profiling of the hematopoietic hierarchy, Proc. Natl. Acad. Sci. U.S.A, vol.107, pp.15443-15448, 2010. ,
Single-molecule detection of epidermal growth factor receptor mutations in plasma by microfluidics digital PCR in non-small cell lung cancer patients, Clin. Cancer Res, vol.15, pp.2076-2084, 2009. ,
Absolute quantitative detection of ABL tyrosine kinase domain point mutations in chronic myeloid leukemia using a novel nanofluidic platform and mutation-specific PCR, Leukemia, vol.23, pp.396-399, 2009. ,
Discovery and prioritization of somatic mutations in diffuse large B-cell lymphoma (DLBCL) by wholeexome sequencing, Proc. Natl. Acad. Sci. U.S.A, vol.109, pp.3879-3884, 2012. ,
Microfluidic amplification as a tool for massive parallel sequencing of the familial hypercholesterolemia genes, Clin. Chem, vol.58, pp.717-724, 2012. ,
A low-cost, high-throughput, automated single nucleotide polymorphism assay for forensic human DNA applications, Anal. Biochem, vol.395, pp.61-67, 2009. ,
High-throughput red blood cell antigen genotyping using a nanofluidic real-time polymerase chain reaction platform, Transfusion, vol.50, pp.40-46, 2010. ,
Development and experimental validation of a predictive threshold cycle equation for quantification of virulence and marker genes by high-throughput nanoliter-volume PCR on the OpenArray platform, Appl. Environ. Microbiol, vol.74, pp.3831-3838, 2008. ,
A high-throughput open-array qPCR gene panel to identify, virulotype, and subtype O157 and non-O157 enterohemorrhagic Escherichia coli, Mol. Cell. Probes, vol.25, pp.222-230, 2011. ,
Megapixel digital PCR, Nat. Methods, vol.8, pp.649-651, 2011. ,
, Digital PCR on a SlipChip. Lab Chip, vol.10, pp.2666-2672, 2010.
Nanoliter multiplex PCR arrays on a SlipChip, Anal. Chem, vol.82, pp.4606-4612, 2010. ,
Multiplexed quantification of nucleic acids with large dynamic range using multivolume digital RT-PCR on a rotational SlipChip tested with HIV and hepatitis C viral load, J. Am. Chem. Soc, vol.133, pp.17705-17712, 2011. ,
Theoretical design and analysis of multivolume digital assays with wide dynamic range validated experimentally with microfluidic digital PCR, Anal. Chem, vol.83, pp.8158-8168, 2011. ,
Detecting changes in the relative expression of KRAS2 splice variants using polymerase colonies, Biotechnol. Prog, vol.20, pp.1836-1839, 2004. ,
Digital genotyping and haplotyping with polymerase colonies, Proc. Natl. Acad. Sci. U.S.A, vol.100, pp.5926-5931, 2003. ,
Long-range polony haplotyping of individual human chromosome molecules, Nat. Genet, vol.38, pp.382-387, 2006. ,
Single molecule profiling of alternative pre-mRNA splicing, Science, vol.301, pp.836-838, 2003. ,
Fluorescent in situ sequencing on polymerase colonies, Anal. Biochem, vol.320, pp.55-65, 2003. ,
Accurate multiplex polony sequencing of an evolved bacterial genome, Science, vol.309, pp.1728-1732, 2005. ,
Man-made cell-like compartments for molecular evolution, Nat. Biotechnol, vol.16, pp.652-656, 1998. ,
A simple method for isolating individual microbes, J. Bacteriol, vol.68, pp.258-259, 1954. ,
Measurement of activity of single molecules of beta-D-galactosidase, Proc. Natl. Acad. Sci. U.S.A, vol.47, 1961. ,
Droplet microfluidics for high-throughput biological assays, Lab Chip, vol.12, pp.2146-2155, 2012. ,
Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology, Angew. Chem. Int. Ed. Engl, vol.49, pp.5846-5868, 2010. ,
Miniaturization and parallelization of biological and chemical assays in microfluidic devices, Chem. Biol, vol.17, pp.1052-1065, 2010. ,
Miniaturizing chemistry and biology in microdroplets, Chem. Commun. (Camb.), vol.18, pp.1773-1788, 2007. ,
URL : https://hal.archives-ouvertes.fr/hal-02148777
Genome sequencing in microfabricated high-density picolitre reactors, Nature, vol.437, pp.376-380, 2005. ,
An integrated semiconductor device enabling non-optical genome sequencing, Nature, vol.475, pp.348-352, 2011. ,
Methods for genomic partitioning, Annu. Rev. Genomics Hum. Genet, vol.10, pp.263-284, 2009. ,
Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations, Proc. Natl. Acad. Sci. U.S.A, vol.100, pp.8817-8822, 2003. ,
BEAMing up for detection and quantification of rare sequence variants, Nat. Methods, vol.3, pp.95-97, 2006. ,
Single-molecule reverse transcription polymerase chain reaction using water-in-oil emulsion, J. Biosci. Bioeng, vol.99, pp.293-295, 2005. ,
PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets, Nucleic Acids Res, vol.33, p.150, 2005. ,
Detection and quantification of mutations in the plasma of patients with colorectal tumors, Proc. Natl. Acad. Sci. U.S.A, vol.102, pp.16368-16373, 2005. ,
Circulating mutant DNA to assess tumor dynamics, Nat. Med, vol.14, pp.985-990, 2008. ,
Analysis of mutations in DNA isolated from plasma and stool of colorectal cancer patients, Gastroenterology, vol.135, pp.489-498, 2008. ,
Detection of tumor PIK3CA status in metastatic breast cancer using peripheral blood, Clin. Cancer Res, 2012. ,
Quantitative detection of EGFR mutations in circulating tumor DNA derived from lung adenocarcinomas, Clin. Cancer Res, vol.17, pp.7808-7815, 2011. ,
Sensitive digital quantification of DNA methylation in clinical samples, Nat. Biotechnol, vol.27, pp.858-863, 2009. ,
The potential of microfluidic water-in-oil droplets in experimental biology, Mol. Biosyst, vol.5, pp.1392-1404, 2009. ,
Microfluidic devices for diagnostic applications, Expert Rev. Mol. Diagn, vol.11, pp.505-519, 2011. ,
Applications of electrowetting-based digital microfluidics in clinical diagnostics, Expert Rev. Mol. Diagn, vol.11, pp.393-407, 2011. ,
Microfluidic droplets: new integrated workflows for biological experiments, Curr. Opin. Chem. Biol, vol.14, pp.548-555, 2010. ,
Droplet microfluidics, Lab Chip, vol.8, pp.198-220, 2008. ,
Single-molecule PCR using water-in-oil emulsion, J. Biotechnol, vol.102, pp.117-124, 2003. ,
Continuous segmented-flow polymerase chain reaction for high-throughput miniaturized DNA amplification, Anal. Chem, vol.75, pp.1-7, 2003. ,
Contamination free continuous flow microfluidic polymerase chain reaction for quantitative and clinical applications, Anal. Chem, vol.77, pp.3700-3704, 2005. ,
On-chip, real-time, single-copy polymerase chain reaction in picoliter droplets, Anal. Chem, vol.79, pp.8471-8475, 2007. ,
High-throughput quantitative polymerase chain reaction in picoliter droplets, Anal. Chem, vol.80, pp.8975-8981, 2008. ,
Continuous-flow polymerase chain reaction of single-copy DNA in microfluidic microdroplets, Anal. Chem, vol.81, pp.302-306, 2009. ,
On-chip single-copy real-time reversetranscription PCR in isolated picoliter droplets, Anal. Chem, vol.80, pp.1854-1858, 2008. ,
1-Million droplet array with wide-field fluorescence imaging for digital PCR, Lab Chip, vol.11, pp.3838-3845, 2011. ,
Tunable 3D droplet self-assembly for ultra-high-density digital micro-reactor arrays, Lab Chip, vol.11, pp.2509-2517, 2011. ,
Quantitative and sensitive detection of rare mutations using droplet-based microfluidics, Lab Chip, vol.11, pp.2156-2166, 2011. ,
URL : https://hal.archives-ouvertes.fr/hal-02148770
Multiplex digital PCR: breaking the one target per color barrier of quantitative PCR, Lab Chip, vol.11, pp.2167-2174, 2011. ,
URL : https://hal.archives-ouvertes.fr/inserm-02299588
Microdroplet-based PCR enrichment for largescale targeted sequencing, Nat. Biotechnol, vol.27, pp.1025-1031, 2009. ,
Detection of low prevalence somatic mutations in solid tumors with ultra-deep targeted sequencing, 2011. ,
, Genome Biol, vol.12, p.124
Sequencing technologies -the next generation, Nat. Rev. Genet, vol.11, pp.31-46, 2010. ,
High-throughput single copy DNA amplification and cell analysis in engineered nanoliter droplets, Anal. Chem, vol.80, pp.3522-3529, 2008. ,
High-performance single cell genetic analysis using microfluidic emulsion generator arrays, Anal. Chem, vol.82, pp.3183-3190, 2010. ,
KRAS mutations as an independent prognostic factor in patients with advanced colorectal cancer treated with cetuximab, J. Clin. Oncol, vol.26, pp.374-379, 2008. ,
URL : https://hal.archives-ouvertes.fr/hal-00363737
High-throughput droplet digital PCR system for absolute quantitation of DNA copy number, Anal. Chem, vol.83, pp.8604-8610, 2011. ,
Droplets for ultrasmall-volume analysis, Anal. Chem, vol.81, pp.5111-5118, 2009. ,
Chemistry and biology in femtoliter and picoliter volume droplets, Acc. Chem. Res, vol.42, pp.649-658, 2009. ,
Droplets as microreactors for high-throughput biology, Chembiochem, vol.8, pp.263-272, 2007. ,
Surfactants in droplet-based microfluidics, Lab Chip, vol.12, pp.422-433, 2012. ,
URL : https://hal.archives-ouvertes.fr/hal-02148769
High-throughput screening of enzymes by retroviral display using droplet-based microfluidics, Chem. Biol, vol.17, pp.229-235, 2010. ,
URL : https://hal.archives-ouvertes.fr/hal-02148765
Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity, Lab Chip, vol.9, pp.1850-1858, 2009. ,
URL : https://hal.archives-ouvertes.fr/hal-02148757
Droplet-based microfluidic platforms for the encapsulation and screening of mammalian cells and multicellular organisms, Chem. Biol, vol.15, pp.427-437, 2008. ,
URL : https://hal.archives-ouvertes.fr/hal-02148746
Dynamic pattern formation in a vesiclegenerating microfluidic device, Phys. Rev. Lett, vol.86, pp.4163-4166, 2001. ,
Microfluidic production of droplet pairs, Langmuir, vol.24, pp.12073-12076, 2008. ,
URL : https://hal.archives-ouvertes.fr/hal-02148748
Droplet-based microreactors for the synthesis of magnetic iron oxide nanoparticles, Angew. Chem. Int. Ed. Engl, vol.47, pp.6817-6820, 2008. ,
URL : https://hal.archives-ouvertes.fr/hal-02148744
A fast and efficient microfluidic system for highly selective one-to-one droplet fusion, Lab Chip, vol.9, pp.2665-2672, 2009. ,
URL : https://hal.archives-ouvertes.fr/hal-02148760
High-throughput injection with microfluidics using picoinjectors, Proc. Natl. Acad. Sci. U.S.A, vol.107, pp.19163-19166, 2010. ,
Droplet-based microfluidic systems for highthroughput single DNA molecule isothermal amplification and analysis, Anal Chem, vol.81, pp.4813-4821, 2009. ,
URL : https://hal.archives-ouvertes.fr/inserm-00420178
An integrated device for monitoring timedependent in vitro expression from single genes in picolitre droplets, Chembiochem, vol.9, pp.439-446, 2008. ,
Reliable microfluidic on-chip incubation of droplets in delay-lines, Lab Chip, vol.9, pp.1344-1348, 2009. ,
Dropspots: a picoliter array in a microfluidic device, Lab Chip, vol.9, pp.44-49, 2009. ,
Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays, Lab Chip, vol.9, pp.692-698, 2009. ,
1-Million droplet array for high-dynamicrange digital microfluidics, Proceedings of the 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, pp.1358-1360, 2010. ,