Digital PCR compartmentalization I. Single-molecule detection of rare mutations.

Karla Perez-Toralla 1 Deniz Pekin 1, 2 Jean-François Bartolo 1 Fanny Garlan 1 Philippe Nizard 1 Pierre Laurent-Puig 1 Jean-Christophe Baret 2, 3 Valérie Taly 1, *
* Corresponding author
1 MEPPOT - U1147 - Médecine Personnalisée, Pharmacogénomique, Optimisation Thérapeutique
2 Droplets membranes and interfaces [Göttingen, Allemagne]
3 CRPP - Centre de recherches Paul Pascal
Abstract : Polymerase chain reaction based techniques have been widely used in laboratory settings. Several applications in oncology, virology or prenatal diagnosis require highly sensitive detection methods, which cannot be achieved with conventional techniques. Digital PCR (dPCR) was developed from the association of PCR and limiting dilution procedures. It is based on the compartmentalization of DNA molecules in small volumes. Controlling the size and the content of each compartment is crucial to obtain a high sensitivity with a single molecule resolution. Microfluidics offers promising tools to isolate DNA fragments such as microdroplets, microchambers or microwells with volumes ranging from few picoliters to nanoliters. The review provides an overview of recent developments of microfluidics dPCR platforms and how this technology can influence the management of cancer patients.
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Karla Perez-Toralla, Deniz Pekin, Jean-François Bartolo, Fanny Garlan, Philippe Nizard, et al.. Digital PCR compartmentalization I. Single-molecule detection of rare mutations.. médecine/sciences, EDP Sciences, 2015, 31 (1), pp.84-92. ⟨10.1051/medsci/20153101017⟩. ⟨inserm-02299576⟩

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