Multiplex Detection of KRAS Mutations Using Passive Droplet Fusion

Abstract : We describe a droplet microfluidics method to screen for multiple mutations of a same oncogene in a single experiment using passive droplet fusion. Genomic DNA from H1573 cell-line was screened for the presence of the six common mutations of the KRAS oncogene as well as wild-type sequences with a detection efficiency of 98 %. Furthermore, the mutant allelic fraction of the cell-line was also assessed correctly showing that the technique is quantitative.
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https://www.hal.inserm.fr/inserm-02299537
Contributor : Valerie Taly <>
Submitted on : Friday, September 27, 2019 - 6:08:08 PM
Last modification on : Wednesday, October 2, 2019 - 1:14:41 AM

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Deniz Pekin, Valérie Taly. Multiplex Detection of KRAS Mutations Using Passive Droplet Fusion. Methods Mol Biol., 1547, pp.133-142, 2017, ⟨10.1007/978-1-4939-6734-6_11⟩. ⟨inserm-02299537⟩

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