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Journal articles
Gelatin-Coated Microfluidic Channels for 3D Microtissue Formation: On-Chip Production and Characterization
Abstract : Traditional two-dimensional (2D) cell culture models are limited in their ability to reproduce human structures and functions. On the contrary, three-dimensional (3D) microtissues have the potential to permit the development of new cell-based assays as advanced in vitro models to test new drugs. Here, we report the use of a dehydrated gelatin film to promote tumor cells aggregation and 3D microtissue formation. The simple and stable gelatin coating represents an alternative to conventional and expensive materials like type I collagen, hyaluronic acid, or matrigel. The gelatin coating is biocompatible with several culture formats including microfluidic chips, as well as standard micro-well plates. It also enables long-term 3D cell culture and in situ monitoring of live/dead assays.
Cited literature [38 references]
https://www.hal.inserm.fr/inserm-02299486
Contributor : Valerie Taly Connect in order to contact the contributor
Submitted on : Friday, September 27, 2019 - 5:37:34 PM
Last modification on : Friday, March 27, 2020 - 2:19:48 AM
Long-term archiving on: : Monday, February 10, 2020 - 3:31:43 AM
Contributor : Valerie Taly Connect in order to contact the contributor
Submitted on : Friday, September 27, 2019 - 5:37:34 PM
Last modification on : Friday, March 27, 2020 - 2:19:48 AM
Long-term archiving on: : Monday, February 10, 2020 - 3:31:43 AM
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micromachines-10-00265.pdf
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