Gelatin-Coated Microfluidic Channels for 3D Microtissue Formation: On-Chip Production and Characterization

Gabriele Pitingolo 1, * Antoine Riaud 1 Claudio Nastruzzi 2 Valérie Taly 1, *
* Corresponding author
1 MEPPOT - U1147 - Médecine Personnalisée, Pharmacogénomique, Optimisation Thérapeutique
2 Dipartimento di Scienze Chimiche e Farmaceutiche [Ferrara, Italy]
Abstract : Traditional two-dimensional (2D) cell culture models are limited in their ability to reproduce human structures and functions. On the contrary, three-dimensional (3D) microtissues have the potential to permit the development of new cell-based assays as advanced in vitro models to test new drugs. Here, we report the use of a dehydrated gelatin film to promote tumor cells aggregation and 3D microtissue formation. The simple and stable gelatin coating represents an alternative to conventional and expensive materials like type I collagen, hyaluronic acid, or matrigel. The gelatin coating is biocompatible with several culture formats including microfluidic chips, as well as standard micro-well plates. It also enables long-term 3D cell culture and in situ monitoring of live/dead assays.
Keywords : 3D microtissues cells viability dehydration gelatin human colon adenocarcinoma hydrogel microfluidics
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Gabriele Pitingolo, Antoine Riaud, Claudio Nastruzzi, Valérie Taly. Gelatin-Coated Microfluidic Channels for 3D Microtissue Formation: On-Chip Production and Characterization. Micromachines, MDPI, 2019, 10 (4), pp.265. ⟨10.3390/mi10040265⟩. ⟨inserm-02299486⟩

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