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Selective Activation of Cognate SNAREpins by Sec1/Munc18 Proteins

Abstract : Sec1/Munc18 (SM) proteins are required for every step of intracellular membrane fusion, but their molecular mechanism of action has been unclear. In this work, we demonstrate a fundamental role of the SM protein: to act as a stimu-latory subunit of its cognate SNARE fusion machinery. In a reconstituted system, mamma-lian SNARE pairs assemble between bilayers to drive a basal fusion reaction. Munc18-1/nSec1, a synaptic SM protein required for neurotrans-mitter release, strongly accelerates this reaction through direct contact with both t-and v-SNAREs. Munc18-1 accelerates fusion only for the cognate SNAREs for exocytosis, therefore enhancing fusion specificity.
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Jingshi Shen, David Tareste, Fabienne Paumet, James Rothman, Thomas Melia. Selective Activation of Cognate SNAREpins by Sec1/Munc18 Proteins. Cell, Elsevier, 2007, 128 (1), pp.183-195. ⟨10.1016/j.cell.2006.12.016⟩. ⟨inserm-02296593⟩



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